Detailed information of TA system TA192455

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 18494..18763
• Replicon: plasmid unnamed
• Accession: NZ_CP069979
• Organism: Escherichia coli strain FDAARGOS_1299

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: I6K29_RS24535
• Protein ID: WP_001372321.1
• Coordinates: 18638..18763 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	18494..18559 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K29_RS24500	13506..13961	-	456	Protein_20	hypothetical protein	-
I6K29_RS24505	14263..14790	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
I6K29_RS24510	14848..15081	+	234	WP_033550308.1	DUF905 family protein	-
I6K29_RS24515	15142..17106	+	1965	WP_033550309.1	ParB/RepB/Spo0J family partition protein	-
I6K29_RS24520	17175..17609	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
I6K29_RS24525	17606..18368	+	763	Protein_25	plasmid SOS inhibition protein A	-
-	18337..18561	+	225	NuclAT_0	-	-
-	18337..18561	+	225	NuclAT_0	-	-
-	18337..18561	+	225	NuclAT_0	-	-
-	18337..18561	+	225	NuclAT_0	-	-
I6K29_RS24530	18346..18525	-	180	WP_001309233.1	hypothetical protein	-
-	18494..18559	-	66	-	-	Antitoxin
I6K29_RS25355	18547..18696	+	150	Protein_27	plasmid maintenance protein Mok	-
I6K29_RS24535	18638..18763	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6K29_RS25360	19064..19361	-	298	Protein_29	hypothetical protein	-
I6K29_RS24540	19661..19957	+	297	WP_001272251.1	hypothetical protein	-
I6K29_RS24545	20067..20888	+	822	WP_001234445.1	DUF932 domain-containing protein	-
I6K29_RS24550	21185..21694	-	510	WP_000759173.1	transglycosylase SLT domain-containing protein	-
I6K29_RS24555	22108..22491	+	384	WP_001151538.1	conjugal transfer relaxosome DNA-binding protein TraM	-
I6K29_RS24560	22678..23367	+	690	WP_033550311.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..99190	99190

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T192455 WP_001372321.1 NZ_CP069979:18638-18763 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T192455 NZ_CP092408:44325-44807 [Klebsiella quasipneumoniae]
GTGGGATGTATAACAGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGAGAAACGGTACTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAGGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATATCTCATTGCACGTAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGCTTCTATGTTCACCATGGATTTAAGGCATCACAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT192455 NZ_CP069979:c18559-18494 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin