Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 727017..727848 Replicon chromosome
Accession NZ_CP069978
Organism Escherichia coli strain FDAARGOS_1299

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag I6K29_RS03490 Protein ID WP_000854814.1
Coordinates 727017..727391 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag I6K29_RS03495 Protein ID WP_001285586.1
Coordinates 727480..727848 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K29_RS03450 (722414) 722414..723579 + 1166 Protein_681 serine-type D-Ala-D-Ala carboxypeptidase DacD -
I6K29_RS03455 (723698) 723698..724171 + 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
I6K29_RS03460 (724369) 724369..725427 + 1059 WP_001200890.1 FUSC family protein -
I6K29_RS03465 (725599) 725599..725928 + 330 WP_000450409.1 DUF496 family protein -
I6K29_RS25135 (726029) 726029..726352 - 324 WP_223216378.1 EutP/PduV family microcompartment system protein -
I6K29_RS25140 (726331) 726331..726411 + 81 WP_023441679.1 hypothetical protein -
I6K29_RS03480 (726700) 726700..726780 - 81 Protein_687 hypothetical protein -
I6K29_RS03485 (726826) 726826..727020 - 195 WP_000988601.1 DUF5983 family protein -
I6K29_RS03490 (727017) 727017..727391 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
I6K29_RS03495 (727480) 727480..727848 - 369 WP_001285586.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
I6K29_RS03500 (727922) 727922..728143 - 222 WP_000692323.1 DUF987 domain-containing protein -
I6K29_RS03505 (728212) 728212..728688 - 477 WP_001298074.1 RadC family protein -
I6K29_RS03510 (728703) 728703..729176 - 474 WP_000855071.1 antirestriction protein -
I6K29_RS03515 (729439) 729439..730260 - 822 WP_001234570.1 DUF932 domain-containing protein -
I6K29_RS03520 (730481) 730481..730891 - 411 WP_000846704.1 hypothetical protein -
I6K29_RS03525 (730907) 730907..731584 - 678 WP_001362823.1 hypothetical protein -
I6K29_RS03530 (731720) 731720..732790 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T192432 WP_000854814.1 NZ_CP069978:c727391-727017 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T192432 NZ_CP092383:c1359652-1358906 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
AGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGGAGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGGACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT192432 WP_001285586.1 NZ_CP069978:c727848-727480 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT192432 NZ_CP092383:c1358905-1358735 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGATAAGTATAAACAAA
AAAAAGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References