Detailed information of TA system TA192391

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 347946..348781
• Replicon: chromosome
• Accession: NZ_CP069973
• Organism: Escherichia coli strain FDAARGOS_1280

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: I6K10_RS02675
• Protein ID: WP_021543420.1
• Coordinates: 348404..348781 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: Q5I3J0
• Locus tag: I6K10_RS02670
• Protein ID: WP_001280951.1
• Coordinates: 347946..348314 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K10_RS02635 (343612)	343612..344496	+	885	WP_000010399.1	50S ribosome-binding GTPase	-
I6K10_RS02640 (344615)	344615..345292	+	678	WP_001097362.1	hypothetical protein	-
I6K10_RS02645 (345298)	345298..345531	+	234	WP_001278288.1	DUF905 family protein	-
I6K10_RS02650 (345621)	345621..346439	+	819	WP_001175170.1	DUF932 domain-containing protein	-
I6K10_RS02655 (346516)	346516..347001	+	486	WP_000213717.1	antirestriction protein	-
I6K10_RS02660 (347017)	347017..347493	+	477	WP_001186724.1	RadC family protein	-
I6K10_RS02665 (347562)	347562..347783	+	222	WP_001405861.1	DUF987 domain-containing protein	-
I6K10_RS02670 (347946)	347946..348314	+	369	WP_001280951.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6K10_RS02675 (348404)	348404..348781	+	378	WP_021543420.1	TA system toxin CbtA family protein	Toxin
I6K10_RS02680 (348778)	348778..348927	+	150	Protein_332	DUF5983 family protein	-
I6K10_RS02685 (349003)	349003..349200	+	198	WP_086795284.1	DUF957 domain-containing protein	-
I6K10_RS02690 (349285)	349285..350127	+	843	WP_001290171.1	DUF4942 domain-containing protein	-
I6K10_RS02695 (350470)	350470..350640	+	171	Protein_335	IS110 family transposase	-
I6K10_RS02700 (351451)	351451..352434	+	984	WP_001298261.1	KpsF/GutQ family sugar-phosphate isomerase	-
I6K10_RS02705 (352506)	352506..353654	+	1149	WP_000905922.1	capsule polysaccharide export inner-membrane protein KpsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13975.96 Da
• Isoelectric Point: 8.2905

>T192391 WP_021543420.1 NZ_CP069973:348404-348781 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T192391 NZ_CP092369:c2185143-2184967 [Bacillus subtilis]
GTGCTTGAGAAAGTGGGTATTATAGTTGCTTTCCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGATAAGAAATCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACATATACGCCGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13699.56 Da
• Isoelectric Point: 7.0268

>AT192391 WP_001280951.1 NZ_CP069973:347946-348314 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT192391 NZ_CP092369:2184926-2185026 [Bacillus subtilis]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATATGTTGGCGTTGTCTCTTTGGTCGGAG
CCGCTTGCGTATACGCTTTTT

Structures

Antitoxin

• AlphaFold DB: Q5I3J0