Detailed information of TA system TA192271

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 43349..43775
• Replicon: plasmid unnamed2
• Accession: NZ_CP069948
• Organism: Klebsiella pneumoniae strain FDAARGOS_1325

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: I6K55_RS26650
• Protein ID: WP_001312861.1
• Coordinates: 43617..43775 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	43349..43573 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K55_RS26625	39216..39743	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
I6K55_RS26630	39801..40034	+	234	WP_000006003.1	DUF905 family protein	-
I6K55_RS26635	40095..42118	+	2024	Protein_52	ParB/RepB/Spo0J family partition protein	-
I6K55_RS26640	42187..42621	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
I6K55_RS26645	42618..43337	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	43349..43573	+	225	NuclAT_0	-	Antitoxin
-	43349..43573	+	225	NuclAT_0	-	Antitoxin
-	43349..43573	+	225	NuclAT_0	-	Antitoxin
-	43349..43573	+	225	NuclAT_0	-	Antitoxin
I6K55_RS26650	43617..43775	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6K55_RS26655	44013..44390	-	378	Protein_56	hypothetical protein	-
I6K55_RS26660	44690..44986	+	297	WP_001272251.1	hypothetical protein	-
I6K55_RS26665	45097..45918	+	822	WP_001234445.1	DUF945 domain-containing protein	-
I6K55_RS26670	46215..46817	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
I6K55_RS26675	47140..47523	+	384	WP_001354030.1	relaxosome protein TraM	-
I6K55_RS26680	47717..48388	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
I6K55_RS26685	48525..48752	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / blaTEM-1B	-	1..85832	85832

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T192271 WP_001312861.1 NZ_CP069948:43617-43775 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T192271 NZ_CP092283:c1051006-1050716 [Marinobacter sp. M4C]
ATGAGCTCTAAGTACGCTCTAGAATTCAAACCCTCTGCCCTTAAAGAATGGAAGAAGCTCGCACCGGCGCTGCAGGCTCA
GTTTAAGAAAAAGCTCAAACAGCGGCTCGACGATCCTCACGTGCCGCCAGACGCCCTATCCGATCTCAAGAACTGCTACA
AAATCAAACTTCGCAGCGCAGGTTACCGATTGGTTTATGAGGTCGAAAACGAGCGCATCGTTGTAACGGTGGTCGCCGTG
GGCAAGCGAGAGCGTGGTGCGGTGTACAGTCGGGCTCACCGACGAATCTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT192271 NZ_CP069948:43349-43573 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin