Detailed information of TA system TA192241

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2891779..2892547
• Replicon: chromosome
• Accession: NZ_CP069945
• Organism: Escherichia coli strain FDAARGOS_1373

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: I6L03_RS13720
• Protein ID: WP_000854814.1
• Coordinates: 2892173..2892547 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: I6L03_RS13715
• Protein ID: WP_071792336.1
• Coordinates: 2891779..2892084 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L03_RS13680 (2886779)	2886779..2887849	+	1071	WP_094283375.1	patatin-like phospholipase family protein	-
I6L03_RS13685 (2887985)	2887985..2888662	+	678	WP_001362823.1	hypothetical protein	-
I6L03_RS13690 (2888678)	2888678..2889088	+	411	WP_000846704.1	hypothetical protein	-
I6L03_RS13695 (2889309)	2889309..2890130	+	822	WP_001234710.1	DUF932 domain-containing protein	-
I6L03_RS13700 (2890393)	2890393..2890866	+	474	WP_204628132.1	antirestriction protein	-
I6L03_RS13705 (2890882)	2890882..2891358	+	477	WP_001186773.1	RadC family protein	-
I6L03_RS13710 (2891421)	2891421..2891642	+	222	WP_000692323.1	DUF987 domain-containing protein	-
I6L03_RS13715 (2891779)	2891779..2892084	+	306	WP_071792336.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6L03_RS13720 (2892173)	2892173..2892547	+	375	WP_000854814.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
I6L03_RS13725 (2892544)	2892544..2892738	+	195	WP_000988601.1	DUF5983 family protein	-
I6L03_RS13730 (2892784)	2892784..2892864	+	81	Protein_2698	hypothetical protein	-
I6L03_RS23120 (2893153)	2893153..2893233	-	81	WP_023441679.1	hypothetical protein	-
I6L03_RS23125 (2893212)	2893212..2893535	+	324	WP_223216378.1	EutP/PduV family microcompartment system protein	-
I6L03_RS13745 (2893636)	2893636..2893965	-	330	WP_000450409.1	DUF496 family protein	-
I6L03_RS13750 (2894137)	2894137..2895195	-	1059	WP_001200894.1	FUSC family protein	-
I6L03_RS13755 (2895393)	2895393..2895866	-	474	WP_001105376.1	DNA gyrase inhibitor SbmC	-
I6L03_RS13760 (2895985)	2895985..2897151	-	1167	WP_001297905.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (2-96)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T192241 WP_000854814.1 NZ_CP069945:2892173-2892547 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T192241 NZ_CP092252:2317768-2318055 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11097.76 Da
• Isoelectric Point: 5.6182

>AT192241 WP_071792336.1 NZ_CP069945:2891779-2892084 [Escherichia coli]
MPCTVTPCFGARLVQEGNRLHYLADRAGIRGLSSDADAYPLDQAFPLLMKQLELMLTSGELNPRNQHTVTLYAKGLACEA
DTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 306 bp

>AT192241 NZ_CP092252:2317529-2317768 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A829FY50