Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1203956..1204518 Replicon chromosome
Accession NZ_CP069935
Organism Escherichia coli strain FDAARGOS_1302

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag I6K32_RS05950 Protein ID WP_000605675.1
Coordinates 1204240..1204518 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6K32_RS05945 Protein ID WP_000781370.1
Coordinates 1203956..1204240 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K32_RS05930 (1199137) 1199137..1202184 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
I6K32_RS05935 (1202197) 1202197..1203081 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
I6K32_RS05940 (1203074) 1203074..1203727 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
I6K32_RS05945 (1203956) 1203956..1204240 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
I6K32_RS05950 (1204240) 1204240..1204518 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K32_RS05955 (1204704) 1204704..1205714 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
I6K32_RS05960 (1205848) 1205848..1207545 - 1698 WP_000433462.1 malate dehydrogenase -
I6K32_RS05965 (1207701) 1207701..1207838 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6K32_RS05970 (1207940) 1207940..1208155 - 216 WP_000495766.1 biofilm-dependent modulation protein -
I6K32_RS05975 (1208500) 1208500..1208931 + 432 WP_000152310.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T192137 WP_000605675.1 NZ_CP069935:c1204518-1204240 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T192137 NZ_CP092077:c4534355-4533822 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT192137 WP_000781370.1 NZ_CP069935:c1204240-1203956 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT192137 NZ_CP092077:c4534632-4534366 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References