Detailed information of TA system TA192090

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4510591..4511375
• Replicon: chromosome
• Accession: NZ_CP069893
• Organism: Escherichia coli strain FDAARGOS_1286

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: I6K16_RS21695
• Protein ID: WP_000613624.1
• Coordinates: 4510591..4511085 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: B1LI61
• Locus tag: I6K16_RS21700
• Protein ID: WP_001110446.1
• Coordinates: 4511082..4511375 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K16_RS21675 (4505784)	4505784..4506881	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
I6K16_RS21680 (4506881)	4506881..4507822	+	942	WP_001317765.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
I6K16_RS21685 (4507888)	4507888..4509531	+	1644	WP_000096482.1	flagellar hook-associated protein FlgK	-
I6K16_RS21690 (4509543)	4509543..4510496	+	954	WP_001212762.1	flagellar hook-associated protein FlgL	-
I6K16_RS21695 (4510591)	4510591..4511085	-	495	WP_000613624.1	GNAT family N-acetyltransferase	Toxin
I6K16_RS21700 (4511082)	4511082..4511375	-	294	WP_001110446.1	DUF1778 domain-containing protein	Antitoxin
I6K16_RS21705 (4511509)	4511509..4514694	-	3186	WP_000827395.1	ribonuclease E	-
I6K16_RS21710 (4515267)	4515267..4516226	+	960	WP_000846329.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (85-145)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18142.04 Da
• Isoelectric Point: 7.7444

>T192090 WP_000613624.1 NZ_CP069893:c4511085-4510591 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIDRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T192090 NZ_CP092065:2254354-2254770 [Edwardsiella ictaluri]
GTGGCCCTTTGGCGCTGTAATGTTCACGCCTCCTGGCGTACCCAGGTGATGTTTCTGCTGGGGCATGGCGCGCTGGTATT
GGCGATCCTGTTGGCGCCCTGGCCGGAAAACTACAGCGTATTCTGGCTGATTCTGCTGGTTGCGGTGATCTTTGAGTGTA
TCCGCAGCCAGCGTCTGATTGCGCGTAACAGCGGTGAGCTACAGCGGCTGGCCCCACAGGTATGGCACTGGCAGCTGCGC
GAGTGGCGCCTGGCCCGGCGCCCCTGGGTTAGCGATCTGGGAGCGTTGCTGATCCTGCAGAGTACCCATGGCACGCCGTG
CCGCCGTCGTCTGTGGCTGGCGGCCGACAGCATGAGCCGCGAAGAGTGGGGGCAGCTGCGCCGCGCGCTGCTCGATACGG
GCGACGATCGCGTATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11079.67 Da
• Isoelectric Point: 10.5423

>AT192090 WP_001110446.1 NZ_CP069893:c4511375-4511082 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT192090 NZ_CP092065:2254107-2254373 [Edwardsiella ictaluri]
ATGGATATCAATAATAAAGCGCGGGTTCACTGGGCGTGCCGCCGCGGAATGCGTGAGCTGGATATCGCCATTATGCCTTT
CTTTGAACATGAGTATGATGCGCTTAACGATGCCGATAAACAGACCTTTGTCCGACTGCTGTCCTGTGACGATCCTGAAC
TATTTAAATGGCTGATGAACCAGGGTAAGCCGGACGATACCGATCTGGCCCGTATGATTACGCTGATTCAGACGCGAAAT
AAAGAACGTGGCCCTTTGGCGCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A829G775