Detailed information of TA system TA192076

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2121754..2122385
• Replicon: chromosome
• Accession: NZ_CP069893
• Organism: Escherichia coli strain FDAARGOS_1286

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L4J012
• Locus tag: I6K16_RS10320
• Protein ID: WP_001260301.1
• Coordinates: 2122110..2122385 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IZA1
• Locus tag: I6K16_RS10315
• Protein ID: WP_000593557.1
• Coordinates: 2121754..2122113 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K16_RS10290 (2117886)	2117886..2118830	+	945	WP_000947063.1	nickel ABC transporter permease subunit NikB	-
I6K16_RS10295 (2118827)	2118827..2119660	+	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
I6K16_RS10300 (2119660)	2119660..2120424	+	765	WP_001136206.1	nickel import ATP-binding protein NikD	-
I6K16_RS10305 (2120421)	2120421..2121227	+	807	WP_000173650.1	nickel import ATP-binding protein NikE	-
I6K16_RS10310 (2121233)	2121233..2121634	+	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
I6K16_RS10315 (2121754)	2121754..2122113	-	360	WP_000593557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
I6K16_RS10320 (2122110)	2122110..2122385	-	276	WP_001260301.1	type II toxin-antitoxin system HicA family toxin	Toxin
I6K16_RS10325 (2122458)	2122458..2123582	-	1125	WP_001318088.1	ABC transporter permease	-
I6K16_RS10330 (2123582)	2123582..2126317	-	2736	WP_000149178.1	ribosome-associated ATPase/putative transporter RbbA	-
I6K16_RS10335 (2126314)	2126314..2127381	-	1068	WP_000361470.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10185.87 Da
• Isoelectric Point: 10.6128

>T192076 WP_001260301.1 NZ_CP069893:c2122385-2122110 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T192076 NZ_CP092055:c1835504-1835352 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGAGAGTCAGTAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13389.28 Da
• Isoelectric Point: 5.1987

>AT192076 WP_000593557.1 NZ_CP069893:c2122113-2121754 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT192076 NZ_CP092055:c1836128-1835529 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATGTAG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
CAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: L4J012

Antitoxin

• AlphaFold DB: L4IZA1