Detailed information of TA system TA192075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 2094514..2095109
• Replicon: chromosome
• Accession: NZ_CP069893
• Organism: Escherichia coli strain FDAARGOS_1286

Toxin (Protein)

• Gene name: doc
• Uniprot ID: D6JG31
• Locus tag: I6K16_RS10165
• Protein ID: WP_000155159.1
• Coordinates: 2094514..2094891 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: D6JG32
• Locus tag: I6K16_RS10170
• Protein ID: WP_000557315.1
• Coordinates: 2094888..2095109 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K16_RS10145 (2090012)	2090012..2091082	-	1071	WP_000907828.1	sn-glycerol 3-phosphate ABC transporter ATP binding protein UgpC	-
I6K16_RS10150 (2091084)	2091084..2091929	-	846	WP_000572164.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
I6K16_RS10155 (2091926)	2091926..2092813	-	888	WP_000099289.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
I6K16_RS10160 (2092989)	2092989..2094305	-	1317	WP_000803191.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
I6K16_RS10165 (2094514)	2094514..2094891	-	378	WP_000155159.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
I6K16_RS10170 (2094888)	2094888..2095109	-	222	WP_000557315.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
I6K16_RS10175 (2095197)	2095197..2095910	-	714	WP_000416895.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
I6K16_RS10180 (2095912)	2095912..2096679	-	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
I6K16_RS10185 (2096676)	2096676..2097953	-	1278	WP_000803825.1	branched chain amino acid ABC transporter permease LivM	-
I6K16_RS10190 (2097950)	2097950..2098876	-	927	WP_000003010.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
I6K16_RS10195 (2098924)	2098924..2100033	-	1110	WP_001318085.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2089272..2105221	15949

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (27-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13964.25 Da
• Isoelectric Point: 6.7263

>T192075 WP_000155159.1 NZ_CP069893:c2094891-2094514 [Escherichia coli]
MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNKYEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILAANPEFVELTVDVAAGKVSLEDIVTRLRQRGT

Nucleotide

• Length: 378 bp

>T192075 NZ_CP092055:c819123-818935 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8103.24 Da
• Isoelectric Point: 6.2168

>AT192075 WP_000557315.1 NZ_CP069893:c2095109-2094888 [Escherichia coli]
MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEFERYQKAKLDAEFDAIMAIHGSEIKELADK

Nucleotide

• Length: 222 bp

>AT192075 NZ_CP092055:c819412-819152 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: D6JG31

Antitoxin

• AlphaFold DB: D6JG32