Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 44572..45173 Replicon plasmid pTA13-2
Accession NZ_CP069714
Organism Escherichia coli strain EA13

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag JSQ77_RS24005 Protein ID WP_001216045.1
Coordinates 44793..45173 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag JSQ77_RS24000 Protein ID WP_001190712.1
Coordinates 44572..44793 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ77_RS23990 41640..42836 - 1197 WP_094316228.1 restriction endonuclease subunit S -
JSQ77_RS23995 42833..44389 - 1557 WP_096314329.1 type I restriction-modification system subunit M -
JSQ77_RS24000 44572..44793 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JSQ77_RS24005 44793..45173 + 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
JSQ77_RS24010 45178..45357 + 180 WP_001339207.1 hypothetical protein -
JSQ77_RS24015 45385..46428 + 1044 WP_069187067.1 DUF968 domain-containing protein -
JSQ77_RS24020 46517..46969 + 453 WP_001326849.1 late promoter-activating protein -
JSQ77_RS24025 47055..48248 + 1194 WP_096321055.1 terminase -
JSQ77_RS24030 48248..49732 + 1485 WP_096321054.1 terminase -
JSQ77_RS24035 49954..50073 + 120 WP_071594056.1 ash family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..93942 93942


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T191774 WP_001216045.1 NZ_CP069714:44793-45173 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T191774 NZ_CP091901:2998326-2998469 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTAAAAGAAAACAATAAAAAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT191774 WP_001190712.1 NZ_CP069714:44572-44793 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT191774 NZ_CP091901:c2998663-2998401 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGATGGTCGTTGTGCTACA
ATGGTAATGAAAAGAGAGGTATGCGCCAACATATCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTTTTCTTTTACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References