Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 160235..160878 Replicon plasmid pTA13-1
Accession NZ_CP069713
Organism Escherichia coli strain EA13

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag JSQ77_RS23645 Protein ID WP_001034044.1
Coordinates 160462..160878 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag JSQ77_RS23640 Protein ID WP_001261286.1
Coordinates 160235..160465 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ77_RS23625 155372..155602 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
JSQ77_RS23630 155599..156015 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
JSQ77_RS23635 156060..159854 - 3795 WP_001144732.1 hypothetical protein -
JSQ77_RS23640 160235..160465 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JSQ77_RS23645 160462..160878 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
JSQ77_RS23650 160953..162518 + 1566 WP_001128474.1 AAA family ATPase -
JSQ77_RS23655 162503..163525 + 1023 WP_000361402.1 helicase UvrD -
JSQ77_RS23660 163779..164476 - 698 Protein_192 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / erm(42) / tet(A) / dfrA14 / mph(A) / aac(3)-IId / blaTEM-1B / sul3 / aph(3')-Ia / sitABCD iutA / iucD / iucC / iucB / iucA 1..182585 182585
- flank IS/Tn - - 163779..164282 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T191773 WP_001034044.1 NZ_CP069713:160462-160878 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T191773 NZ_CP091901:c2991537-2991196 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT191773 WP_001261286.1 NZ_CP069713:160235-160465 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT191773 NZ_CP091901:c2991767-2991537 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GCCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References