Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 155372..156015 Replicon plasmid pTA13-1
Accession NZ_CP069713
Organism Escherichia coli strain EA13

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag JSQ77_RS23630 Protein ID WP_001034046.1
Coordinates 155599..156015 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag JSQ77_RS23625 Protein ID WP_001261278.1
Coordinates 155372..155602 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ77_RS23595 150882..151187 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
JSQ77_RS23600 151189..151407 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
JSQ77_RS23605 151975..152487 + 513 WP_000151784.1 hypothetical protein -
JSQ77_RS23610 152521..153654 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
JSQ77_RS23615 153821..154594 - 774 WP_000905949.1 hypothetical protein -
JSQ77_RS23620 154607..155107 - 501 WP_000528931.1 hypothetical protein -
JSQ77_RS23625 155372..155602 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JSQ77_RS23630 155599..156015 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
JSQ77_RS23635 156060..159854 - 3795 WP_001144732.1 hypothetical protein -
JSQ77_RS23640 160235..160465 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
JSQ77_RS23645 160462..160878 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / erm(42) / tet(A) / dfrA14 / mph(A) / aac(3)-IId / blaTEM-1B / sul3 / aph(3')-Ia / sitABCD iutA / iucD / iucC / iucB / iucA 1..182585 182585


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T191772 WP_001034046.1 NZ_CP069713:155599-156015 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T191772 NZ_CP091901:c2918045-2917902 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT191772 WP_001261278.1 NZ_CP069713:155372-155602 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT191772 NZ_CP091901:2917782-2917969 [Enterococcus faecalis]
TGTGCTATAATGAAAACGAAAAGAGAGATATGCTTCAACATACCTCTCTGATGCAGAACCGTTTAAGACGGTGACTTTTT
GAATTATTTAAAAATAACCGTACTCGGTCAAAGTTGACGGTTATTTTTTATTGTCATTTTTAAGCAATTTCACAATCAGT
GCAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References