Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1918342..1918883 Replicon chromosome
Accession NZ_CP069646
Organism Escherichia coli O7:H4 strain MIN14

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag JSU15_RS09475 Protein ID WP_000615981.1
Coordinates 1918605..1918883 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag JSU15_RS09470 Protein ID WP_000729704.1
Coordinates 1918342..1918602 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU15_RS09450 (1914025) 1914025..1914810 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
JSU15_RS09455 (1914782) 1914782..1916494 + 1713 Protein_1880 flagellar biosynthesis protein FlhA -
JSU15_RS09460 (1916710) 1916710..1917207 - 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
JSU15_RS09465 (1917383) 1917383..1918156 - 774 WP_001353765.1 C40 family peptidase -
JSU15_RS09470 (1918342) 1918342..1918602 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JSU15_RS09475 (1918605) 1918605..1918883 + 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JSU15_RS09480 (1919039) 1919039..1919779 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JSU15_RS09485 (1919750) 1919750..1920517 - 768 WP_000333379.1 class II glutamine amidotransferase -
JSU15_RS09490 (1920723) 1920723..1921301 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T191505 WP_000615981.1 NZ_CP069646:1918605-1918883 [Escherichia coli O7:H4]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T191505 NZ_CP091756:c2591947-2591771 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT191505 WP_000729704.1 NZ_CP069646:1918342-1918602 [Escherichia coli O7:H4]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT191505 NZ_CP091756:c2591725-2591309 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References