Detailed information of TA system TA191479

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1801753..1802587
• Replicon: chromosome
• Accession: NZ_CP069642
• Organism: Escherichia coli strain SSGNw2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: JSQ95_RS08490
• Protein ID: WP_000854690.1
• Coordinates: 1802210..1802587 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JSQ95_RS08485
• Protein ID: WP_119023792.1
• Coordinates: 1801753..1802121 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSQ95_RS08445	1796835..1797962	+	1128	Protein_1656	hypothetical protein	-
JSQ95_RS08450	1798038..1798493	+	456	WP_000581502.1	IrmA family protein	-
JSQ95_RS08455	1798572..1798805	+	234	WP_000902034.1	DUF905 family protein	-
JSQ95_RS08460	1798906..1799724	+	819	WP_001234620.1	DUF932 domain-containing protein	-
JSQ95_RS08465	1799779..1800264	+	486	WP_000849565.1	antirestriction protein	-
JSQ95_RS08470	1800280..1800756	+	477	WP_001186726.1	RadC family protein	-
JSQ95_RS08475	1800819..1801040	+	222	WP_000692329.1	DUF987 domain-containing protein	-
JSQ95_RS08480	1801059..1801703	+	645	WP_000094916.1	hypothetical protein	-
JSQ95_RS08485	1801753..1802121	+	369	WP_119023792.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
JSQ95_RS08490	1802210..1802587	+	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
JSQ95_RS08495	1802584..1803072	+	489	WP_000761699.1	DUF5983 family protein	-
JSQ95_RS08500	1803089..1803286	+	198	WP_000839293.1	DUF957 domain-containing protein	-
JSQ95_RS08505	1803371..1804216	+	846	WP_001529401.1	DUF4942 domain-containing protein	-
JSQ95_RS08510	1804285..1804680	+	396	WP_000208382.1	DUF6088 family protein	-
JSQ95_RS08515	1804673..1805606	+	934	Protein_1670	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JSQ95_RS08520	1806023..1806193	+	171	Protein_1671	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	kpsF / kpsE / kpsD / kpsU / kpsC / kpsS / ugd / kpsT	1801753..1823796	22043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T191479 WP_000854690.1 NZ_CP069642:1802210-1802587 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T191479 NZ_CP091712:c4554698-4554591 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13601.51 Da
• Isoelectric Point: 4.5851

>AT191479 WP_119023792.1 NZ_CP069642:1801753-1802121 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT191479 NZ_CP091712:4554755-4554810 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Structures

Toxin

• AlphaFold DB: S1PLF5