Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3359959..3360794 Replicon chromosome
Accession NZ_CP069639
Organism Escherichia coli strain SSGNw1

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0J2AEA6
Locus tag JSQ92_RS15940 Protein ID WP_000854759.1
Coordinates 3360417..3360794 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag JSQ92_RS15935 Protein ID WP_001295723.1
Coordinates 3359959..3360327 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ92_RS15910 (3357074) 3357074..3357269 + 196 Protein_3107 DUF905 family protein -
JSQ92_RS15915 (3357387) 3357387..3358205 + 819 WP_001234738.1 DUF932 domain-containing protein -
JSQ92_RS15920 (3358547) 3358547..3359020 + 474 WP_001350782.1 antirestriction protein -
JSQ92_RS15925 (3359036) 3359036..3359512 + 477 WP_001186775.1 RadC family protein -
JSQ92_RS15930 (3359575) 3359575..3359796 + 222 WP_000692345.1 DUF987 domain-containing protein -
JSQ92_RS15935 (3359959) 3359959..3360327 + 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
JSQ92_RS15940 (3360417) 3360417..3360794 + 378 WP_000854759.1 TA system toxin CbtA family protein Toxin
JSQ92_RS15945 (3360791) 3360791..3361279 + 489 WP_000761690.1 DUF5983 family protein -
JSQ92_RS15950 (3361296) 3361296..3361472 + 177 WP_000839286.1 DUF957 domain-containing protein -
JSQ92_RS15955 (3361578) 3361578..3361727 + 150 Protein_3116 hypothetical protein -
JSQ92_RS15960 (3362094) 3362094..3362270 + 177 Protein_3117 helix-turn-helix domain-containing protein -
JSQ92_RS15965 (3363061) 3363061..3364683 + 1623 WP_001295726.1 Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3346265..3373693 27428


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14144.26 Da        Isoelectric Point: 7.3249

>T191456 WP_000854759.1 NZ_CP069639:3360417-3360794 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T191456 NZ_CP091705:c5526-5251 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT191456 WP_001295723.1 NZ_CP069639:3359959-3360327 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT191456 NZ_CP091705:c5810-5526 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2AEA6

Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References