Detailed information of TA system TA191382

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4684596..4685397
• Replicon: chromosome
• Accession: NZ_CP069630
• Organism: Escherichia coli strain ESM2.2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0H2V687
• Locus tag: JSQ94_RS22240
• Protein ID: WP_000854739.1
• Coordinates: 4685017..4685397 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0P7R0L9
• Locus tag: JSQ94_RS22235
• Protein ID: WP_001285482.1
• Coordinates: 4684596..4684970 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSQ94_RS22200 (4680684)	4680684..4681139	+	456	WP_000581502.1	IrmA family protein	-
JSQ94_RS22205 (4681282)	4681282..4681371	+	90	WP_230607454.1	DUF905 family protein	-
JSQ94_RS22210 (4681550)	4681550..4682371	+	822	WP_001234710.1	DUF932 domain-containing protein	-
JSQ94_RS22215 (4682634)	4682634..4683107	+	474	WP_000855076.1	antirestriction protein	-
JSQ94_RS22220 (4683123)	4683123..4683599	+	477	WP_001186170.1	RadC family protein	-
JSQ94_RS22225 (4683662)	4683662..4683883	+	222	WP_000692312.1	DUF987 domain-containing protein	-
JSQ94_RS22230 (4683902)	4683902..4684546	+	645	WP_000094915.1	hypothetical protein	-
JSQ94_RS22235 (4684596)	4684596..4684970	+	375	WP_001285482.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JSQ94_RS22240 (4685017)	4685017..4685397	+	381	WP_000854739.1	TA system toxin CbtA family protein	Toxin
JSQ94_RS22245 (4685394)	4685394..4685882	+	489	WP_001054232.1	DUF5983 family protein	-
JSQ94_RS22250 (4685902)	4685902..4686099	+	198	WP_000772027.1	DUF957 domain-containing protein	-
JSQ94_RS22255 (4686184)	4686184..4687027	+	844	Protein_4347	DUF4942 domain-containing protein	-
JSQ94_RS22265 (4687498)	4687498..4688436	+	939	WP_000351287.1	glyoxylate/hydroxypyruvate reductase GhrA	-
JSQ94_RS22270 (4688491)	4688491..4689228	+	738	WP_000283664.1	zinc-binding phosphatase	-
JSQ94_RS22275 (4689252)	4689252..4689806	+	555	WP_001001909.1	molecular chaperone YcdY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4659828..4685397	25569

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14214.14 Da
• Isoelectric Point: 6.4773

>T191382 WP_000854739.1 NZ_CP069630:4685017-4685397 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNDITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T191382 NZ_CP091700:427737-427910 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13791.44 Da
• Isoelectric Point: 4.7511

>AT191382 WP_001285482.1 NZ_CP069630:4684596-4684970 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT191382 NZ_CP091700:427413-427709 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGACTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0H2V687

Antitoxin

• AlphaFold DB: A0A0P7R0L9