Detailed information of TA system TA191375

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3359959..3360794
• Replicon: chromosome
• Accession: NZ_CP069630
• Organism: Escherichia coli strain ESM2.2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: JSQ94_RS15945
• Protein ID: WP_000854759.1
• Coordinates: 3360417..3360794 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: JSQ94_RS15940
• Protein ID: WP_001295723.1
• Coordinates: 3359959..3360327 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSQ94_RS15915 (3357074)	3357074..3357269	+	196	Protein_3107	DUF905 family protein	-
JSQ94_RS15920 (3357387)	3357387..3358205	+	819	WP_001234738.1	DUF932 domain-containing protein	-
JSQ94_RS15925 (3358547)	3358547..3359020	+	474	WP_001350782.1	antirestriction protein	-
JSQ94_RS15930 (3359036)	3359036..3359512	+	477	WP_001186775.1	RadC family protein	-
JSQ94_RS15935 (3359575)	3359575..3359796	+	222	WP_000692345.1	DUF987 domain-containing protein	-
JSQ94_RS15940 (3359959)	3359959..3360327	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JSQ94_RS15945 (3360417)	3360417..3360794	+	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
JSQ94_RS15950 (3360791)	3360791..3361279	+	489	WP_000761690.1	DUF5983 family protein	-
JSQ94_RS15955 (3361296)	3361296..3361472	+	177	WP_000839286.1	DUF957 domain-containing protein	-
JSQ94_RS15960 (3361578)	3361578..3361727	+	150	Protein_3116	hypothetical protein	-
JSQ94_RS15965 (3362094)	3362094..3362270	+	177	Protein_3117	helix-turn-helix domain-containing protein	-
JSQ94_RS15970 (3363061)	3363061..3364683	+	1623	WP_001295726.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3346265..3373693	27428

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T191375 WP_000854759.1 NZ_CP069630:3360417-3360794 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T191375 NZ_CP091691:c4418104-4417727 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT191375 WP_001295723.1 NZ_CP069630:3359959-3360327 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT191375 NZ_CP091691:c4418562-4418194 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTAACAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52