Detailed information of TA system TA191298

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3547593..3548143
• Replicon: chromosome
• Accession: NZ_CP069621
• Organism: Escherichia coli strain PBP5

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PDG4
• Locus tag: JSQ88_RS16865
• Protein ID: WP_001160966.1
• Coordinates: 3547829..3548143 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: JSQ88_RS16860
• Protein ID: WP_000125566.1
• Coordinates: 3547593..3547826 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSQ88_RS16835 (3542666)	3542666..3542953	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
JSQ88_RS16840 (3543012)	3543012..3544343	+	1332	WP_001183207.1	MFS transporter	-
JSQ88_RS16845 (3544451)	3544451..3544981	+	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
JSQ88_RS16850 (3544974)	3544974..3546836	+	1863	WP_000377123.1	glutathione-regulated potassium-efflux system protein KefC	-
JSQ88_RS16855 (3547028)	3547028..3547507	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
JSQ88_RS16860 (3547593)	3547593..3547826	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
JSQ88_RS16865 (3547829)	3547829..3548143	+	315	WP_001160966.1	CcdB family protein	Toxin
JSQ88_RS16870 (3548140)	3548140..3548988	-	849	WP_000257182.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
JSQ88_RS16875 (3548995)	3548995..3549372	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
JSQ88_RS16880 (3549375)	3549375..3550196	-	822	WP_001065363.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
JSQ88_RS16885 (3550193)	3550193..3551182	-	990	WP_000241229.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
JSQ88_RS16890 (3551182)	3551182..3552468	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.72 Da
• Isoelectric Point: 9.0851

>T191298 WP_001160966.1 NZ_CP069621:3547829-3548143 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T191298 NZ_CP091673:c3672363-3671956 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT191298 WP_000125566.1 NZ_CP069621:3547593-3547826 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT191298 NZ_CP091673:c3672610-3672344 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A829G815

Antitoxin

• AlphaFold DB: A0A7U9LTM9