Detailed information of TA system TA191056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3525225..3525827
• Replicon: chromosome
• Accession: NZ_CP069589
• Organism: Escherichia coli strain FDAARGOS_1266

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6J96_RS17325
• Protein ID: WP_000897302.1
• Coordinates: 3525225..3525536 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6J96_RS17330
• Protein ID: WP_000356397.1
• Coordinates: 3525537..3525827 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J96_RS17300	3521139..3521738	+	600	WP_001296610.1	glucose-1-phosphatase	-
I6J96_RS17305	3521732..3522604	+	873	WP_000920762.1	virulence factor BrkB family protein	-
I6J96_RS17310	3522601..3523038	+	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I6J96_RS17315	3523083..3524024	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6J96_RS17320	3524088..3524996	-	909	WP_001385591.1	alpha/beta hydrolase	-
I6J96_RS17325	3525225..3525536	+	312	WP_000897302.1	hypothetical protein	Toxin
I6J96_RS17330	3525537..3525827	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
I6J96_RS17335	3526186..3526464	+	279	WP_001296612.1	hypothetical protein	-
I6J96_RS17340	3526861..3527079	+	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
I6J96_RS17345	3527264..3527713	-	450	WP_032140890.1	hypothetical protein	-
I6J96_RS17350	3528029..3528877	-	849	WP_001038650.1	hypothetical protein	-
I6J96_RS17355	3529167..3529409	+	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
I6J96_RS17360	3529591..3530520	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T191056 WP_000897302.1 NZ_CP069589:3525225-3525536 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T191056 NZ_CP091626:c4457472-4457161 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT191056 WP_000356397.1 NZ_CP069589:3525537-3525827 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT191056 NZ_CP091626:c4457160-4456870 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGGTATTGA

Structures

Toxin

• AlphaFold DB: S1P416