Detailed information of TA system TA191052

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2498537..2499371
• Replicon: chromosome
• Accession: NZ_CP069589
• Organism: Escherichia coli strain FDAARGOS_1266

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: I6J96_RS12400
• Protein ID: WP_000854690.1
• Coordinates: 2498994..2499371 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1P7N8
• Locus tag: I6J96_RS12395
• Protein ID: WP_001305076.1
• Coordinates: 2498537..2498905 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J96_RS12355	2493619..2494746	+	1128	Protein_2427	hypothetical protein	-
I6J96_RS12360	2494822..2495277	+	456	WP_000581502.1	hypothetical protein	-
I6J96_RS12365	2495356..2495589	+	234	WP_000902034.1	DUF905 family protein	-
I6J96_RS12370	2495690..2496508	+	819	WP_001234620.1	DUF945 domain-containing protein	-
I6J96_RS12375	2496563..2497048	+	486	WP_000849565.1	antirestriction protein	-
I6J96_RS12380	2497064..2497540	+	477	WP_001186726.1	RadC family protein	-
I6J96_RS12385	2497603..2497824	+	222	WP_000692329.1	DUF987 domain-containing protein	-
I6J96_RS12390	2497843..2498487	+	645	WP_000094916.1	hypothetical protein	-
I6J96_RS12395	2498537..2498905	+	369	WP_001305076.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6J96_RS12400	2498994..2499371	+	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
I6J96_RS12405	2499368..2499856	+	489	WP_000761699.1	hypothetical protein	-
I6J96_RS12410	2499873..2500070	+	198	WP_000839293.1	DUF957 domain-containing protein	-
I6J96_RS12415	2500155..2501000	+	846	WP_001529401.1	DUF4942 domain-containing protein	-
I6J96_RS12420	2501069..2501464	+	396	WP_000208382.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
I6J96_RS12425	2501457..2502390	+	934	Protein_2441	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
I6J96_RS12430	2502822..2502977	+	156	WP_000729638.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	kpsF / kpsE / kpsD / kpsU / kpsC / kpsS / kpsT	2454259..2516944	62685
	-	flank	IS/Tn	-	-	2502822..2502977	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T191052 WP_000854690.1 NZ_CP069589:2498994-2499371 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T191052 NZ_CP091626:1973234-1973337 [Salmonella enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13560.39 Da
• Isoelectric Point: 4.7830

>AT191052 WP_001305076.1 NZ_CP069589:2498537-2498905 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT191052 NZ_CP091626:c1973339-1973196 [Salmonella enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Structures

Toxin

• AlphaFold DB: S1PLF5

Antitoxin

• AlphaFold DB: S1P7N8