Detailed information of TA system TA190914

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3352499..3353294
• Replicon: chromosome
• Accession: NZ_CP069559
• Organism: Escherichia coli strain FDAARGOS_1259

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: I6J89_RS16315
• Protein ID: WP_000854914.1
• Coordinates: 3352499..3352873 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7UP42
• Locus tag: I6J89_RS16320
• Protein ID: WP_001280955.1
• Coordinates: 3352920..3353294 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J89_RS16275	3347504..3347995	-	492	WP_001340063.1	DUF1097 domain-containing protein	-
I6J89_RS16280	3348097..3348651	-	555	WP_001001921.1	molecular chaperone YcdY	-
I6J89_RS16285	3348675..3349412	-	738	WP_000283664.1	zinc-binding phosphatase	-
I6J89_RS16290	3349467..3350405	-	939	WP_000351277.1	glyoxylate/hydroxypyruvate reductase GhrA	-
I6J89_RS16300	3350875..3351717	-	843	WP_001280481.1	DUF4942 domain-containing protein	-
I6J89_RS16305	3351802..3351999	-	198	WP_000839282.1	DUF957 domain-containing protein	-
I6J89_RS16310	3352011..3352502	-	492	WP_000976857.1	hypothetical protein	-
I6J89_RS16315	3352499..3352873	-	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
I6J89_RS16320	3352920..3353294	-	375	WP_001280955.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6J89_RS16325	3353457..3353678	-	222	WP_001220314.1	DUF987 domain-containing protein	-
I6J89_RS16330	3353741..3354217	-	477	WP_001186738.1	RadC family protein	-
I6J89_RS16335	3354233..3354718	-	486	WP_000214398.1	antirestriction protein	-
I6J89_RS16340	3354809..3355627	-	819	WP_001234682.1	DUF945 domain-containing protein	-
I6J89_RS16345	3355967..3357037	-	1071	WP_000102669.1	patatin-like phospholipase family protein	-
I6J89_RS16350	3357034..3357939	-	906	WP_023149949.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	csgB / csgD / csgE / csgF / csgG	3343887..3354718	10831

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T190914 WP_000854914.1 NZ_CP069559:c3352873-3352499 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T190914 NZ_CP091571:2478678-2478962 [Salmonella enterica]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13767.59 Da
• Isoelectric Point: 6.6248

>AT190914 WP_001280955.1 NZ_CP069559:c3353294-3352920 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT190914 NZ_CP091571:2478440-2478688 [Salmonella enterica]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5

Antitoxin

• AlphaFold DB: A0A7U9P0D0