Detailed information of TA system TA190600

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3407607..3408439
• Replicon: chromosome
• Accession: NZ_CP069480
• Organism: Escherichia coli strain FDAARGOS_1257

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: I6J87_RS16420
• Protein ID: WP_000854753.1
• Coordinates: 3407607..3407981 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: I6J87_RS16425
• Protein ID: WP_001531802.1
• Coordinates: 3408071..3408439 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J87_RS16390	3402722..3403870	-	1149	WP_000905914.1	capsule polysaccharide transporter	-
I6J87_RS16395	3403942..3404925	-	984	WP_001335608.1	KpsF/GutQ family sugar-phosphate isomerase	-
I6J87_RS16400	3405735..3405905	-	171	Protein_3199	IS110 family transposase	-
I6J87_RS16405	3406247..3406816	-	570	WP_001290247.1	DUF4942 domain-containing protein	-
I6J87_RS16410	3406913..3407110	-	198	WP_000839281.1	DUF957 domain-containing protein	-
I6J87_RS16415	3407122..3407610	-	489	WP_000777541.1	hypothetical protein	-
I6J87_RS16420	3407607..3407981	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
I6J87_RS16425	3408071..3408439	-	369	WP_001531802.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6J87_RS16430	3408602..3408823	-	222	WP_000692329.1	DUF987 domain-containing protein	-
I6J87_RS16435	3408886..3409362	-	477	WP_001186726.1	RadC family protein	-
I6J87_RS16440	3409378..3409863	-	486	WP_001531954.1	antirestriction protein	-
I6J87_RS16445	3409918..3410739	-	822	WP_001531953.1	DUF945 domain-containing protein	-
I6J87_RS16450	3410820..3411031	+	212	Protein_3209	hypothetical protein	-
I6J87_RS16455	3411150..3411605	-	456	WP_000581502.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3405735..3405839	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T190600 WP_000854753.1 NZ_CP069480:c3407981-3407607 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T190600 NZ_CP091482:c236664-236377 [Enterobacter cloacae complex sp. ECL414]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13422.27 Da
• Isoelectric Point: 6.3161

>AT190600 WP_001531802.1 NZ_CP069480:c3408439-3408071 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNSLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT190600 NZ_CP091482:c236903-236664 [Enterobacter cloacae complex sp. ECL414]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0