Detailed information of TA system TA190593

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1733522..1734357
• Replicon: chromosome
• Accession: NZ_CP069480
• Organism: Escherichia coli strain FDAARGOS_1257

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1R2L4
• Locus tag: I6J87_RS08440
• Protein ID: WP_001094426.1
• Coordinates: 1733522..1733899 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: Q1R2L5
• Locus tag: I6J87_RS08445
• Protein ID: WP_001285575.1
• Coordinates: 1733989..1734357 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J87_RS08415	1729025..1729204	+	180	WP_001298063.1	hypothetical protein	-
I6J87_RS08420	1729603..1731639	-	2037	WP_000417001.1	hypothetical protein	-
I6J87_RS08425	1732591..1732743	-	153	WP_011478345.1	hypothetical protein	-
I6J87_RS08430	1732828..1733025	-	198	WP_000839272.1	DUF957 domain-containing protein	-
I6J87_RS08435	1733037..1733525	-	489	WP_000761683.1	hypothetical protein	-
I6J87_RS08440	1733522..1733899	-	378	WP_001094426.1	TA system toxin CbtA family protein	Toxin
I6J87_RS08445	1733989..1734357	-	369	WP_001285575.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6J87_RS08450	1734437..1734658	-	222	WP_000692350.1	DUF987 domain-containing protein	-
I6J87_RS08455	1734745..1735221	-	477	WP_001298074.1	RadC family protein	-
I6J87_RS08460	1735236..1735721	-	486	WP_000213697.1	antirestriction protein	-
I6J87_RS08465	1735812..1736630	-	819	WP_001175160.1	DUF945 domain-containing protein	-
I6J87_RS08470	1736720..1736953	-	234	WP_001278290.1	DUF905 family protein	-
I6J87_RS08475	1736959..1737636	-	678	WP_001097301.1	hypothetical protein	-
I6J87_RS08480	1737784..1738464	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	1720235..1750190	29955

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14072.99 Da
• Isoelectric Point: 7.3523

>T190593 WP_001094426.1 NZ_CP069480:c1733899-1733522 [Escherichia coli]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T190593 NZ_CP091481:c1171680-1171318 [Enterobacter cloacae complex sp. ECL414]
ATGTGGAATGTCGAGACAACGGACATATTTGATCAATGGTACTTCGAACAAACAACAGCATTAAAAGAAGATGTGCTAGC
GATGATGCATATTCTTGCTGAGTTTGGCCCAACGCTTGGGCGGCCATACGTAGATACCGTGAAGGCTTCAGCCTATGCGA
ACATGAAGGAATTACGGATTCAACATGCGGGAAATCCCATCAGAGCTTTTTTTGCTTTTGATCCTGACCGTAAGGCAATT
GTGCTCTGTGCTGGAGACAAAACCGGTTGCAATCAAAAACGGTTTTATAACGAAATGATTACGCTGGCTGACGTTGAGTA
CAGCAGACATCTGGCGGATAAGGAGGCCATATGGCAACGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13709.52 Da
• Isoelectric Point: 6.6258

>AT190593 WP_001285575.1 NZ_CP069480:c1734357-1733989 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT190593 NZ_CP091481:c1171330-1171031 [Enterobacter cloacae complex sp. ECL414]
ATGGCAACGCTAAAAGAACTCATGGCACAACAGAGTGCGGAAAGTCAGGAGCGTATTGCGGCTAAAGTTGATGTAATACG
CAGAATTGTTGCCCTGAACCAACTGCGGGAAGAGCTCAATATTTCGCAGACAGAACTGGCCGCGGCAATGGGGGTAAAAC
AACCAACTGTGGCAAAAATCGAACAGCCGGGAAACGATCCCCGGCTTTCAACACTCAAACGCTATGTCAGTGCGCTCGGC
GGTGAAATGAGCATTGATGTGACGTTACCGAATGGGAAACGAATAGCGTTTCAGATTTGA

Structures

Toxin

• AlphaFold DB: A0A454ABD6

Antitoxin

• AlphaFold DB: A0A454ABF5