Detailed information of TA system TA190584

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1218737..1219572
• Replicon: chromosome
• Accession: NZ_CP069480
• Organism: Escherichia coli strain FDAARGOS_1257

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q83W73
• Locus tag: I6J87_RS05960
• Protein ID: WP_000854700.1
• Coordinates: 1218737..1219114 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: I6J87_RS05965
• Protein ID: WP_001278232.1
• Coordinates: 1219204..1219572 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J87_RS05930	1214059..1214553	+	495	WP_032143180.1	MarR family transcriptional regulator	-
I6J87_RS05935	1214988..1215326	-	339	WP_000772639.1	Arm DNA-binding domain-containing protein	-
I6J87_RS05940	1215692..1217011	+	1320	WP_000144686.1	site-specific integrase	-
I6J87_RS05945	1217104..1217958	-	855	WP_024174313.1	DUF4942 domain-containing protein	-
I6J87_RS05950	1218043..1218240	-	198	WP_000839247.1	DUF957 domain-containing protein	-
I6J87_RS05955	1218252..1218740	-	489	WP_000761726.1	hypothetical protein	-
I6J87_RS05960	1218737..1219114	-	378	WP_000854700.1	TA system toxin CbtA family protein	Toxin
I6J87_RS05965	1219204..1219572	-	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
I6J87_RS05970	1219735..1219956	-	222	WP_000692353.1	DUF987 domain-containing protein	-
I6J87_RS05975	1220025..1220501	-	477	WP_001186199.1	RadC family protein	-
I6J87_RS05980	1220517..1221002	-	486	WP_000213723.1	antirestriction protein	-
I6J87_RS05985	1221094..1221912	-	819	WP_001234709.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE / vat	1170802..1247474	76672

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14276.28 Da
• Isoelectric Point: 7.8046

>T190584 WP_000854700.1 NZ_CP069480:c1219114-1218737 [Escherichia coli]
MKTLPDTHVREASRCLSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SPCTRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T190584 NZ_CP091470:c4825337-4825080 [Klebsiella michiganensis]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCACATCCGCGATGCATC
AGGCCGCGTCACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAGCTGATTAAGTCCGTCAACGGACAGCCCTACCGCATCAATACCACCGGGCTGAATAACGTCCGCGCA
CAGCTGGACAATCGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT190584 WP_001278232.1 NZ_CP069480:c1219572-1219204 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT190584 NZ_CP091470:c4825069-4824518 [Klebsiella michiganensis]
ATGACAAACCATAGCTTCACTTTTCATGTCGCCTCTGAAGGCGATACCGATGACATTCTCGACGTCGAAACCCGGGCTTT
CGGCTACAGCAAAGAGGCCAGGCTGGTGGCCGATTTGCTCAACGATGAGAGCGCCTGCCCGACGCTATCGCTGCTGGCCA
GGCATAACGGAGAGGCGGTTGGGCATATTTTATTCACCCGGGCGACGTTCAAAGGGGAGCCGGACTCGCCGATGATGCAC
ATCCTTGCTCCCCTGGCGGTGGTGCCGGAATACCAGGGCGTGGGCGTTGGCGGAGAGCTTATCCGGCACGGCATTGAGCA
GTTGAAAGCAATGGGAAGCCAGATGGTATTTGTTCTGGGCCACGCCGCTTACTACCCCAGGCACGGGTTTGAGCCATGCG
CGGGAGATAAAGGCTATCCGGCGCCTTATCCGATTCCGGAGGCGCATAAAGCCTGCTGGATGCTGCAGCGGCTTTCGTCT
CAGCCTCTGGGACGAACGGGCCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Structures

Toxin

• AlphaFold DB: A0A454A1I2

Antitoxin

• AlphaFold DB: E3PJ72