Detailed information of TA system TA190339

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 490252..490889
• Replicon: chromosome
• Accession: NZ_CP069430
• Organism: Bacillus velezensis strain TB918

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: JQN69_RS02465
• Protein ID: WP_003156187.1
• Coordinates: 490539..490889 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: JQN69_RS02460
• Protein ID: WP_003156188.1
• Coordinates: 490252..490533 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQN69_RS02440	486617..487216	-	600	WP_015239195.1	rhomboid family intramembrane serine protease	-
JQN69_RS02445	487309..487674	+	366	WP_094031521.1	holo-ACP synthase	-
JQN69_RS02450	487839..488846	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
JQN69_RS02455	488963..490132	+	1170	WP_025284272.1	alanine racemase	-
JQN69_RS02460	490252..490533	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
JQN69_RS02465	490539..490889	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
JQN69_RS02470	491007..491828	+	822	WP_003156182.1	STAS domain-containing protein	-
JQN69_RS02475	491833..492198	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
JQN69_RS02480	492201..492602	+	402	WP_003156178.1	anti-sigma regulatory factor	-
JQN69_RS02485	492614..493621	+	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
JQN69_RS02490	493685..494014	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
JQN69_RS02495	494011..494493	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
JQN69_RS02500	494459..495247	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
JQN69_RS02505	495247..495849	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T190339 WP_003156187.1 NZ_CP069430:490539-490889 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T190339 NZ_CP091299:c66744-66346 [Salmonella enterica subsp. enterica serovar Bovismorbificans]
ATGCTGAAGTTTATGCTGGATACCAATATCTGTATCTTTACGATAAAAAACAAACCTGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATTTACGGCGCCGAAAAAAGCCGGATGCCCG
AAAGAAATCTCGCTGTGATAGAGGGGTTTGTTTCCCGACTTGATGTTCTGGATTATGACGCGCCGGCCGCCACCCATACG
GGGCAGATCAGGGCAGAGCTTGCCCGTCAGGGACGCCCTGTCGGGCCATTCGATCAGATGATTGCCGGTCACGCACGCAG
CCGGGGACTGATTGTTGTCACGAACAACACCCGGGAGTTTGAACGTGTGGGTGGACTGAGAACTGAGGACTGGAGCTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT190339 WP_003156188.1 NZ_CP069430:490252-490533 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT190339 NZ_CP091299:c66971-66744 [Salmonella enterica subsp. enterica serovar Bovismorbificans]
ATGGAAACCAGCGTATTTCTCAGTAACCGAAGCCAGGCGGTCAGACTGCCAAAAGCAGTGGCATTGCCGGAACATGTAAA
ACGTGTGGAAGTTATCGCTGTCGGGCGAACCCGGATCATCACGCCAGCCGGAGAATCGTGGGATGAATGGTTCGACGGGA
ACAACGTCAGCGCCGATTTTATGGATAACCGGGAACAACCCTCTGTGCAGGAACGGGAATCATTCTGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5