189930

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 2759273..2760710 Replicon chromosome
Accession NZ_CP069132
Organism Escherichia coli BW25113 substr. CHXR1G20

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag JQK35_RS13425 Protein ID WP_000155570.1
Coordinates 2759273..2760346 (+) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag JQK35_RS13430 Protein ID WP_000461704.1
Coordinates 2760339..2760710 (+) Length 124 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JQK35_RS13410 2754706..2756895 - 2190 WP_000136402.1 DEAD/DEAH box helicase -
JQK35_RS13415 2756892..2758508 - 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
JQK35_RS13420 2758868..2759131 - 264 WP_000502050.1 DUF1524 domain-containing protein -
JQK35_RS13425 2759273..2760346 + 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
JQK35_RS13430 2760339..2760710 + 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
JQK35_RS13435 2761065..2761928 + 864 WP_000787597.1 GTPase family protein -
JQK35_RS13440 2762020..2762841 + 822 WP_000197391.1 DUF945 domain-containing protein -
JQK35_RS13445 2763058..2763759 + 702 WP_000189409.1 WYL domain-containing protein -
JQK35_RS13450 2763800..2764036 + 237 WP_001144029.1 hypothetical protein -
JQK35_RS13455 2764036..2764479 + 444 WP_000824222.1 hypothetical protein -
JQK35_RS13460 2764503..2764970 + 468 WP_000702495.1 hypothetical protein -
JQK35_RS13465 2765195..2765509 - 315 WP_000065803.1 arsenate reductase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T189930 WP_000155570.1 NZ_CP069132:2759273-2760346 [Escherichia coli BW25113]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T189930 NZ_CP091027:95716-96114 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT189930 WP_000461704.1 NZ_CP069132:2760339-2760710 [Escherichia coli BW25113]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT189930 NZ_CP091027:95489-95716 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S


Antitoxin

Source ID Structure
AlphaFold DB A0A373F4G4

References