Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1274185..1274963 Replicon chromosome
Accession NZ_CP069084
Organism Xanthomonas campestris pv. campestris strain GSXT20191014

Toxin (Protein)


Gene name TacT2 Uniprot ID Q8P667
Locus tag JOA57_RS05305 Protein ID WP_011038220.1
Coordinates 1274185..1274676 (-) Length 164 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID Q8P668
Locus tag JOA57_RS05310 Protein ID WP_011038219.1
Coordinates 1274673..1274963 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JOA57_RS05290 (JOA57_05285) 1270513..1271388 + 876 WP_011038222.1 30S ribosomal protein S6--L-glutamate ligase -
JOA57_RS05295 (JOA57_05290) 1272038..1272715 + 678 WP_003490678.1 response regulator transcription factor -
JOA57_RS05300 (JOA57_05295) 1272708..1274042 + 1335 WP_019237347.1 HAMP domain-containing sensor histidine kinase -
JOA57_RS05305 (JOA57_05300) 1274185..1274676 - 492 WP_011038220.1 GNAT family N-acetyltransferase Toxin
JOA57_RS05310 (JOA57_05305) 1274673..1274963 - 291 WP_011038219.1 DUF1778 domain-containing protein Antitoxin
JOA57_RS05315 (JOA57_05310) 1275038..1275439 - 402 WP_019237348.1 SymE family type I addiction module toxin -
JOA57_RS05320 (JOA57_05315) 1275971..1276594 - 624 WP_011038217.1 dephospho-CoA kinase -
JOA57_RS05325 (JOA57_05320) 1276608..1277471 - 864 WP_011038216.1 A24 family peptidase -
JOA57_RS05330 (JOA57_05325) 1277478..1278734 - 1257 WP_011038215.1 type II secretion system F family protein -
JOA57_RS05335 (JOA57_05330) 1279061..1279501 + 441 WP_011038214.1 pilin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1244295..1277771 33476


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-144)

Antitoxin

Vibrio phage ICP1, Orf50

(1-95)


Sequences

Toxin        


Download         Length: 164 a.a.        Molecular weight: 17638.31 Da        Isoelectric Point: 8.1192

>T189897 WP_011038220.1 NZ_CP069084:c1274676-1274185 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Download         Length: 492 bp

>T189897 NZ_CP091024:2869390-2869497 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10678.23 Da        Isoelectric Point: 10.5480

>AT189897 WP_011038219.1 NZ_CP069084:c1274963-1274673 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Download         Length: 291 bp

>AT189897 NZ_CP091024:c2869334-2869278 [Escherichia coli]
TCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q8P667

Antitoxin

Source ID Structure
AlphaFold DB Q8P668

References