Detailed information of TA system TA189639

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3796629..3797158
• Replicon: chromosome
• Accession: NZ_CP069074
• Organism: Mycobacterium tuberculosis strain N1274

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: JN139_RS17880
• Protein ID: WP_003417760.1
• Coordinates: 3796901..3797158 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: JN139_RS17875
• Protein ID: WP_003417757.1
• Coordinates: 3796629..3796904 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN139_RS17840	3793202..3793891	-	690	WP_003917774.1	BBE domain-containing protein	-
JN139_RS17845	3794078..3794449	-	372	WP_003417739.1	FAD-binding protein	-
JN139_RS17850	3794347..3794565	-	219	WP_157132559.1	hypothetical protein	-
JN139_RS17855	3794592..3794852	-	261	WP_003417741.1	hypothetical protein	-
JN139_RS17860	3794967..3795356	+	390	WP_003417745.1	DUF732 domain-containing protein	-
JN139_RS17865	3795370..3795663	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
JN139_RS17870	3795660..3796505	-	846	WP_003417751.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
JN139_RS17875	3796629..3796904	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
JN139_RS17880	3796901..3797158	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
JN139_RS17885	3797200..3798390	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
JN139_RS17890	3798507..3798874	+	368	Protein_3533	FHA domain-containing protein	-
JN139_RS17895	3798871..3799422	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
JN139_RS17900	3799429..3800010	-	582	WP_003417769.1	ATP/GTP-binding protein	-
JN139_RS17905	3799991..3800359	-	369	WP_003417772.1	DUF742 domain-containing protein	-
JN139_RS17910	3800337..3800729	-	393	WP_003417776.1	serine protease inhibitor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T189639 WP_003417760.1 NZ_CP069074:3796901-3797158 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T189639 NZ_CP090908:c3479121-3478867 [Serratia marcescens]
ATGAACAAACGACACCAAAAAACGCTGTCAGATGTGTTTGCCCGGCCTGTCAACGGTTCTATAAAGTGGTCTGATATTGA
GGCCCTTTTTACCGCATTAGGGGCGGAGATTCATGAAAGGGAAGGTTCTAGGATCGCGGTGTTGTTGAAAGGTGAAAAAA
GAGTCTTTCATCGGCCACACCCCAGACCTACCACTGACAAGGGGGCGGTTAACTCCATTCGGATCTGGTTGGATAGTTTA
GGAATAAAACCATGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT189639 WP_003417757.1 NZ_CP069074:3796629-3796904 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT189639 NZ_CP090908:c3478870-3478526 [Serratia marcescens]
ATGATGAACAACACACTGAAAATTGGCGGCCATACGGCCGTCATCAACTTCGACCCTGAAATTGAAATGTTCCGGGGTGA
GTTTGTCGGGCTGAACGGTGGCGCCGACTTCTACGCCTACAGCGTGGACGAGCTGAAGAAAGAAGGCGCGCTCTCACTCG
CGGTCTTTCTGGATGAGTGCCAAAAAGACGGCATCGAGCCTTACAAGTCGTATAGCGGCAAAGTAACCACCCGTTTGTCG
CCGGAACGCCATCAGGCGCTAGCCCTTGCCGCACAGGCGACCGGGCAGTCTATTAATGAGCTGCTGAATGAGGGTGTTGA
TTTAGTTATTGAAAAACATTCCTGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• PDB: 3CTO
• PDB: 3OEI
• PDB: 3D55
• AlphaFold DB: G0TI94