Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2333447..2334083 Replicon chromosome
Accession NZ_CP069063
Organism Mycobacterium tuberculosis strain N3913

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag JN150_RS10885 Protein ID WP_003410654.1
Coordinates 2333673..2334083 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag JN150_RS10880 Protein ID WP_003410651.1
Coordinates 2333447..2333680 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN150_RS10865 2328895..2329296 + 402 WP_009937711.1 metal ABC transporter permease -
JN150_RS10870 2329297..2329701 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
JN150_RS10875 2329785..2333369 - 3585 WP_050069428.1 cobaltochelatase subunit CobN -
JN150_RS10880 2333447..2333680 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
JN150_RS10885 2333673..2334083 + 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
JN150_RS10890 2334067..2335158 + 1092 WP_003900454.1 precorrin-3B synthase -
JN150_RS10895 2335168..2335794 + 627 WP_003410658.1 precorrin-8X methylmutase -
JN150_RS10900 2335791..2337317 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
JN150_RS10905 2337263..2338486 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T188960 WP_003410654.1 NZ_CP069063:2333673-2334083 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T188960 NZ_CP090596:c26631-26245 [Pectobacterium aroidearum]
ATGGAGGTTTACAGATTGGCATGGGAAATAGTCACACGGGCGCTATTCGATACGTGGTTTGATGAGCAAACCGAAACGGT
ACAGGAAGAGGTTTTGGCACACCTCGGTATACTCGAAGAAGATGGCCCACAGCTAGGGCGACCACAGGTTGATCAGATTA
AAGGCTCACAATACAAAAATATGAAGGAGCTTCGAGTTCAGGTTGGCGGCCACCCATTCCGAACATTCTTCGCGTTTGAT
CGGACACGTAAAGCGATTGTTTTATGTGCGGGAGATAAGAAAGGCGAAAATGAAAAATTGTTCTACGAACGTATGATTAA
GATCGCAGACGCTGAGTTTACATCGCATCTGATATCACCGGAGGTAAAAGAAGATGGCGACATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT188960 WP_003410651.1 NZ_CP069063:2333447-2333680 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT188960 NZ_CP090596:c26258-25959 [Pectobacterium aroidearum]
ATGGCGACATTTAAAGAAATGATGGCTAAACGTTCACCGGAGAGCCGCGCCCGCATTGAAGCGCGCACGGCTGAGATCCG
TCAGGAAATCACTTTAGCGAAACTACGCGAAGAGCTGAACATGTCGCAGACTCAGCTTGCATTAGCTCTGGGCGTCAAGC
AGCCATCTATTGTTAAGATGGAGAAGGTCGAGAATGACCCAAAACTGTCAACGCTGAAACGCTATGTCACAGCCCTAGGC
GGCAAGCTTACGCTAGATGTGACCTTGCCTGATGGTAAGCGTATCGCGCTTACTTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References