Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2274423..2275342 Replicon chromosome
Accession NZ_CP069063
Organism Mycobacterium tuberculosis strain N3913

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag JN150_RS10660 Protein ID WP_003900449.1
Coordinates 2274737..2275342 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag JN150_RS10655 Protein ID WP_003410124.1
Coordinates 2274423..2274728 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN150_RS10625 2269434..2270690 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -
JN150_RS10630 2271044..2271619 + 576 WP_003410100.1 hypothetical protein -
JN150_RS10635 2271616..2272656 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
JN150_RS10640 2272898..2273617 + 720 WP_003410108.1 DUF433 domain-containing protein -
JN150_RS10645 2273607..2274023 + 417 WP_003410114.1 hypothetical protein -
JN150_RS10650 2274039..2274338 - 300 WP_003410120.1 hypothetical protein -
JN150_RS10655 2274423..2274728 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
JN150_RS10660 2274737..2275342 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JN150_RS10665 2275367..2275726 - 360 WP_003410131.1 hypothetical protein -
JN150_RS10670 2275886..2276476 - 591 WP_057145698.1 hypothetical protein -
JN150_RS10675 2276515..2276751 - 237 WP_003410133.1 hypothetical protein -
JN150_RS10680 2276958..2277854 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T188959 WP_003900449.1 NZ_CP069063:c2275342-2274737 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T188959 NZ_CP090595:4903820-4904107 [Pectobacterium aroidearum]
ATGAGCTATAGCGTCAAATTCAGGGAAGATGCGCTGAAAGAATGGCTCAAGCTCGATAAAACCATCCAGCAGCAATTTGC
CAAAAAACTAAAAAAATGCTGCGAAAATCCCCATATTCCTTCGGCAAAATTACGGGGGATGAAGGACTGCTACAAAATAA
AGCTCCGAGCATCAGGCTTTCGTTTGGTTTATGAAGTCATTGACGATGTATTGATTATCGCCGTAGTGGCGGTAGGTAAA
CGTGAACGCAGTGGCGTTTATCATCTGGCGAGTGAGCGAATGAGATAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT188959 WP_003410124.1 NZ_CP069063:c2274728-2274423 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT188959 NZ_CP090595:4903572-4903823 [Pectobacterium aroidearum]
ATGCCGAATATTATATTGAGTGATACCAGTGCCAGCGTCAGTGAATTGAAAAAGAACCCGATGGCGACGGTCAATGCTGG
CGACGGTCTACCTGTCGCTATTTTGAACCGCAACCAGCCTGCGTTTTATTGCATTCCTGCGGATCTCTACGAAAAAATGC
TGGATGCGCTGGACGATCAAGAACTGGTTAAGCTAATCGGTGAACGCAAAAACCAGCCGCTGGTTGACGTGGATTTGGAT
AGTTTCTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References