Detailed information of TA system TA188943

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1239949..1240517
• Replicon: chromosome
• Accession: NZ_CP069063
• Organism: Mycobacterium tuberculosis strain N3913

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: JN150_RS05940
• Protein ID: WP_003405865.1
• Coordinates: 1240143..1240517 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: JN150_RS05935
• Protein ID: WP_003405863.1
• Coordinates: 1239949..1240146 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN150_RS05915	1235990..1236628	-	639	WP_003898730.1	lipid droplet-associated protein	-
JN150_RS05920	1236718..1237725	+	1008	WP_050069416.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
JN150_RS05925	1237742..1238725	-	984	WP_003898731.1	hypothetical protein	-
JN150_RS05930	1238788..1239861	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
JN150_RS05935	1239949..1240146	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN150_RS05940	1240143..1240517	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN150_RS05945	1240720..1241418	+	699	WP_003898733.1	hypothetical protein	-
JN150_RS05950	1241536..1241721	+	186	WP_003901093.1	hypothetical protein	-
JN150_RS05955	1241648..1241923	-	276	WP_003405867.1	hypothetical protein	-
JN150_RS05960	1242166..1242489	+	324	WP_003405871.1	putative quinol monooxygenase	-
JN150_RS05965	1242504..1243202	-	699	WP_031646953.1	hypothetical protein	-
JN150_RS05970	1243236..1243445	+	210	WP_003911400.1	hypothetical protein	-
JN150_RS05975	1243397..1244167	-	771	Protein_1177	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T188943 WP_003405865.1 NZ_CP069063:1240143-1240517 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T188943 NZ_CP090594:c3972573-3972160 [Pectobacterium aroidearum]
GTGGCCCAGTGGCAATGTGATCTTCGCGTTTCCTGGCGTATGCAACTGCTTTCATTGATTGTGCATGGCCTATTAGTATT
GCTCATTCTTCTGGCGCCGTGGCCGGACGGCTATGCATGGCTGTGGCTATGCCTGGTCACGATGGTGATGTTCGGTTTTA
TCCGTAGCCAAAGGAATATCAAATCCCGGCAGGGGGAAATCGTTTTGCTCAGCGAAACGACCCTGAACTGGCGGCAGCAG
GAATGGCAGATCGTTAAACGACCGTGGTTGCTGAAAAATGGCGTATTGCTGTCATTGCAGGCAGTGAACGGTAAAGACAG
ACAACAGCTGTGGCTGGCATCGGACAGCATGGGCGATGACGAATGGCGTCATCTGCGTCAGCTTCTTTTGCAGCAGAAAA
ACTGGGCGAGATAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT188943 WP_003405863.1 NZ_CP069063:1239949-1240146 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT188943 NZ_CP090594:c3972820-3972554 [Pectobacterium aroidearum]
ATGGAAATCGATAATAAATCACGCATTCATTGGGCATGCCGCCGGGGTATGCGTGAGTTGGATATCGCGATCATGCCGTT
TTTTGAGCATGACTACGACACACTCAGTGACAGCGATAAGCGCACGTTTGTGCGCCTGTTACAAAGCGACGATCCCGATT
TATTCAACTGGCTGATGAACCACGGCGAGCCGAAAGACGGAGAGCTGAAACGCATGATTTCTCTTATTCAAACGCGAAAT
AAAGATCGTGGCCCAGTGGCAATGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07