Detailed information of TA system TA188683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1661771..1662498
• Replicon: chromosome
• Accession: NZ_CP068827
• Organism: Escherichia coli strain RIVM_C030371

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: JNN58_RS07945
• Protein ID: WP_000547555.1
• Coordinates: 1662187..1662498 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN58_RS07940
• Protein ID: WP_000126294.1
• Coordinates: 1661771..1662190 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN58_RS07920	1656856..1657383	-	528	WP_001078777.1	electron transport protein HydN	-
JNN58_RS07925	1657532..1658542	-	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
JNN58_RS07930	1658802..1660259	+	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
JNN58_RS07935	1660268..1661692	+	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
JNN58_RS07940	1661771..1662190	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
JNN58_RS07945	1662187..1662498	-	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
JNN58_RS07950	1662665..1663135	-	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
JNN58_RS07955	1663128..1663538	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
JNN58_RS07960	1663535..1664302	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
JNN58_RS07965	1664302..1664844	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
JNN58_RS07970	1664854..1666563	-	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T188683 WP_000547555.1 NZ_CP068827:c1662498-1662187 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T188683 NZ_CP090533:c4985235-4984924 [Salmonella enterica]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATACTATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT188683 WP_000126294.1 NZ_CP068827:c1662190-1661771 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT188683 NZ_CP090533:c4984927-4984481 [Salmonella enterica]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG

Structures

Toxin

• AlphaFold DB: V0YLE2