Detailed information of TA system TA188637

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4705030..4705642
• Replicon: chromosome
• Accession: NZ_CP068825
• Organism: Escherichia coli strain RIVM_C030256

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: JNN79_RS22655
• Protein ID: WP_000833473.1
• Coordinates: 4705457..4705642 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: JNN79_RS22650
• Protein ID: WP_000499744.1
• Coordinates: 4705030..4705440 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN79_RS22635	4700542..4701357	-	816	WP_000891825.1	AraC family transcriptional regulator	-
JNN79_RS22640	4701583..4702983	+	1401	WP_000204802.1	MFS transporter	-
JNN79_RS22645	4702994..4704958	+	1965	WP_001026861.1	glycoside hydrolase family 127 protein	-
JNN79_RS22650	4705030..4705440	-	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JNN79_RS22655	4705457..4705642	-	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
JNN79_RS22660	4705920..4707458	-	1539	WP_001309845.1	aldehyde dehydrogenase AldB	-
JNN79_RS22665	4707659..4708810	-	1152	WP_000741501.1	L-threonine dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T188637 WP_000833473.1 NZ_CP068825:c4705642-4705457 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T188637 NZ_CP090518:1400335-1400601 [Xylella fastidiosa subsp. fastidiosa]
TTGGCCTGGACGATTGATTACACCGACACGGCCAAGCAGCAGTTACGCAAGCTCGACAAGCACATGGCGCGGCGCATTGT
CGATTTCATGGATGAGCGCATCGCCGGACTAGAAAACCCACGCAGCAGTGGTAAAGCATTAACCGGACCACTTGGCGGCT
TTTGGCGTTATCGTGTTGGTGATTTCCGGGTCGTCTGCGCCATTCAAGATAGCGTTTTGCGCGTCCTTGTAGTGCGTGTT
GGTCACCGGGGTGAGATTTACCGATAG

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT188637 WP_000499744.1 NZ_CP068825:c4705440-4705030 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT188637 NZ_CP090518:1400124-1400351 [Xylella fastidiosa subsp. fastidiosa]
ATGGCGACTTCGATTCGCCTCTCTCCTGAAATGGAGCAAAGGCTGAACTCTCTTGCCTCCCATACGGGACGCACAAAGGC
TTACTACCTACGTGAAATCATTGAGCATGGCATTGAGGAAATGGAGGATTACTACCTTGCTGCCGATGTGTTGGAACGTG
TCCGTCATGGACAAGAACAGGTGCATTCTGCTGCCGATGTGAGGAAAACGCTTGGCCTGGACGATTGA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9