Detailed information of TA system TA188381

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4397076..4397488
• Replicon: chromosome
• Accession: NZ_CP068817
• Organism: Escherichia coli strain RIVM_C028786

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: JNN59_RS21135
• Protein ID: WP_000132614.1
• Coordinates: 4397147..4397488 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4397076..4397152 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN59_RS21125	4393751..4395220	+	1470	WP_001540814.1	type I restriction-modification system subunit M	-
JNN59_RS21130	4395220..4396926	+	1707	WP_001100194.1	restriction endonuclease subunit S	-
-	4397076..4397152	-	77	NuclAT_6	-	Antitoxin
-	4397076..4397152	-	77	NuclAT_6	-	Antitoxin
-	4397076..4397152	-	77	NuclAT_6	-	Antitoxin
-	4397076..4397152	-	77	NuclAT_6	-	Antitoxin
-	4397076..4397152	-	77	NuclAT_7	-	Antitoxin
-	4397076..4397152	-	77	NuclAT_7	-	Antitoxin
-	4397076..4397152	-	77	NuclAT_7	-	Antitoxin
-	4397076..4397152	-	77	NuclAT_7	-	Antitoxin
JNN59_RS21135	4397147..4397488	+	342	WP_000132614.1	endoribonuclease SymE	Toxin
JNN59_RS21140	4397535..4399628	-	2094	WP_000648237.1	DUF262 domain-containing protein	-
JNN59_RS21145	4399726..4399806	-	81	WP_020233658.1	hypothetical protein	-
JNN59_RS21150	4400034..4400954	-	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
JNN59_RS21155	4401139..4402419	+	1281	WP_001298033.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	4380955..4399691	18736

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T188381 WP_000132614.1 NZ_CP068817:4397147-4397488 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T188381 NZ_CP090429:812823-813233 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT188381 NZ_CP068817:c4397152-4397076 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin