Detailed information of TA system TA188293

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1959591..1960422
• Replicon: chromosome
• Accession: NZ_CP068815
• Organism: Escherichia coli strain RIVM_C028620

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: JNN49_RS09175
• Protein ID: WP_000854814.1
• Coordinates: 1959591..1959965 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JNN49_RS09180
• Protein ID: WP_024174331.1
• Coordinates: 1960054..1960422 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN49_RS09135	1954987..1956153	+	1167	WP_001297905.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
JNN49_RS09140	1956272..1956745	+	474	WP_001105376.1	DNA gyrase inhibitor SbmC	-
JNN49_RS09145	1956943..1958001	+	1059	WP_001200890.1	FUSC family protein	-
JNN49_RS09150	1958173..1958502	+	330	WP_000450409.1	DUF496 family protein	-
JNN49_RS09155	1958603..1958785	-	183	WP_001016348.1	hypothetical protein	-
JNN49_RS09160	1958839..1958985	+	147	Protein_1793	transposase domain-containing protein	-
JNN49_RS09165	1959274..1959387	-	114	WP_001161660.1	DUF957 domain-containing protein	-
JNN49_RS09170	1959400..1959594	-	195	WP_000988601.1	hypothetical protein	-
JNN49_RS09175	1959591..1959965	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
JNN49_RS09180	1960054..1960422	-	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN49_RS09185	1960496..1960717	-	222	WP_000692346.1	DUF987 domain-containing protein	-
JNN49_RS09190	1960786..1961262	-	477	WP_001541535.1	RadC family protein	-
JNN49_RS09195	1961278..1961751	-	474	WP_000855074.1	antirestriction protein	-
JNN49_RS09200	1962014..1962835	-	822	WP_001234571.1	DUF945 domain-containing protein	-
JNN49_RS09205	1963056..1963466	-	411	WP_000846704.1	hypothetical protein	-
JNN49_RS09210	1963482..1964159	-	678	WP_001362823.1	hypothetical protein	-
JNN49_RS09215	1964295..1965365	-	1071	WP_000102631.1	patatin-like phospholipase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T188293 WP_000854814.1 NZ_CP068815:c1959965-1959591 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T188293 NZ_CP090394:53928-54410 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT188293 WP_024174331.1 NZ_CP068815:c1960422-1960054 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT188293 NZ_CP090394:53674-53940 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A829FY50