Detailed information of TA system TA188262

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2032058..2032889
• Replicon: chromosome
• Accession: NZ_CP068814
• Organism: Escherichia coli strain RIVM_C028613

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: JNN56_RS09580
• Protein ID: WP_000854814.1
• Coordinates: 2032058..2032432 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B3Y195
• Locus tag: JNN56_RS09585
• Protein ID: WP_001285585.1
• Coordinates: 2032521..2032889 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN56_RS09540	2027454..2028620	+	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
JNN56_RS09545	2028739..2029212	+	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
JNN56_RS09550	2029410..2030468	+	1059	WP_001200891.1	FUSC family protein	-
JNN56_RS09555	2030640..2030969	+	330	WP_000450409.1	DUF496 family protein	-
JNN56_RS09560	2031070..2031252	-	183	WP_001016348.1	hypothetical protein	-
JNN56_RS09565	2031324..2031452	+	129	Protein_1874	transposase domain-containing protein	-
JNN56_RS09570	2031741..2031854	-	114	WP_001161660.1	DUF957 domain-containing protein	-
JNN56_RS09575	2031867..2032061	-	195	WP_000988600.1	hypothetical protein	-
JNN56_RS09580	2032058..2032432	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
JNN56_RS09585	2032521..2032889	-	369	WP_001285585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
JNN56_RS09590	2032963..2033184	-	222	WP_000692323.1	DUF987 domain-containing protein	-
JNN56_RS09595	2033247..2033723	-	477	WP_001186726.1	RadC family protein	-
JNN56_RS09600	2033739..2034218	-	480	WP_000860087.1	antirestriction protein	-
JNN56_RS09605	2034300..2035118	-	819	WP_001234629.1	DUF945 domain-containing protein	-
JNN56_RS09610	2035218..2035451	-	234	WP_001119719.1	DUF905 family protein	-
JNN56_RS09615	2035530..2035985	-	456	WP_005051844.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T188262 WP_000854814.1 NZ_CP068814:c2032432-2032058 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T188262 NZ_CP090386:1021046-1021324 [Vibrio cholerae]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCCGC
TGAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATGGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.48 Da
• Isoelectric Point: 6.3139

>AT188262 WP_001285585.1 NZ_CP068814:c2032889-2032521 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT188262 NZ_CP090386:1020765-1021049 [Vibrio cholerae]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGTCAGGGTCGGAC
TCTTAGTGATGCTTGTCGTGAACTCACTGAACAGCTGGCAGAGCAACAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A7U9LW60