Detailed information of TA system TA188224

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5004165..5005009
• Replicon: chromosome
• Accession: NZ_CP068812
• Organism: Escherichia coli strain RIVM_C029020

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: JNN61_RS23580
• Protein ID: WP_000854686.1
• Coordinates: 5004626..5005009 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: JNN61_RS23575
• Protein ID: WP_001285602.1
• Coordinates: 5004165..5004545 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN61_RS23540	5000414..5000869	+	456	WP_001504120.1	hypothetical protein	-
JNN61_RS23545	5000948..5001181	+	234	WP_001119729.1	DUF905 family protein	-
JNN61_RS23550	5001281..5002099	+	819	WP_001234732.1	DUF945 domain-containing protein	-
JNN61_RS23555	5002191..5002676	+	486	WP_000214307.1	antirestriction protein	-
JNN61_RS23560	5002692..5003168	+	477	WP_001186726.1	RadC family protein	-
JNN61_RS23565	5003231..5003452	+	222	WP_000692298.1	DUF987 domain-containing protein	-
JNN61_RS23570	5003471..5004154	+	684	WP_000086768.1	hypothetical protein	-
JNN61_RS23575	5004165..5004545	+	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN61_RS23580	5004626..5005009	+	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
JNN61_RS23585	5005006..5005494	+	489	WP_001054233.1	hypothetical protein	-
JNN61_RS23590	5005511..5005708	+	198	WP_000839253.1	DUF957 domain-containing protein	-
JNN61_RS23595	5005793..5006635	+	843	WP_001431817.1	DUF4942 domain-containing protein	-
JNN61_RS23605	5007106..5008044	+	939	WP_001550942.1	glyoxylate/hydroxypyruvate reductase GhrA	-
JNN61_RS23610	5008099..5008836	+	738	WP_000283664.1	zinc-binding phosphatase	-
JNN61_RS23615	5008860..5009414	+	555	WP_001001909.1	molecular chaperone YcdY	-
JNN61_RS23620	5009516..5010007	+	492	WP_032145787.1	DUF1097 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T188224 WP_000854686.1 NZ_CP068812:5004626-5005009 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T188224 NZ_CP090375:c2176257-2175895 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCGGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT188224 WP_001285602.1 NZ_CP068812:5004165-5004545 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT188224 NZ_CP090375:c2176424-2176254 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA