Detailed information of TA system TA188215

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3406690..3407522
• Replicon: chromosome
• Accession: NZ_CP068812
• Organism: Escherichia coli strain RIVM_C029020

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: JNN61_RS16100
• Protein ID: WP_000854765.1
• Coordinates: 3406690..3407064 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: JNN61_RS16105
• Protein ID: WP_001295723.1
• Coordinates: 3407154..3407522 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN61_RS16080	3403054..3404592	-	1539	WP_001187178.1	lysine decarboxylation/transport transcriptional activator CadC	-
JNN61_RS16085	3405341..3406183	-	843	WP_022645115.1	DUF4942 domain-containing protein	-
JNN61_RS16090	3406268..3406462	-	195	WP_024181941.1	DUF957 domain-containing protein	-
JNN61_RS16095	3406487..3406690	-	204	WP_001398797.1	hypothetical protein	-
JNN61_RS16100	3406690..3407064	-	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
JNN61_RS16105	3407154..3407522	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN61_RS16110	3407685..3407906	-	222	WP_000692341.1	DUF987 domain-containing protein	-
JNN61_RS16115	3407969..3408445	-	477	WP_001186774.1	RadC family protein	-
JNN61_RS16120	3408461..3408940	-	480	WP_057109541.1	antirestriction protein	-
JNN61_RS16125	3409206..3410024	-	819	WP_001175165.1	DUF945 domain-containing protein	-
JNN61_RS16130	3410114..3410347	-	234	WP_001278293.1	DUF905 family protein	-
JNN61_RS16135	3410353..3411030	-	678	WP_001097302.1	hypothetical protein	-
JNN61_RS16140	3411178..3411858	-	681	WP_001282921.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3395565..3441323	45758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T188215 WP_000854765.1 NZ_CP068812:c3407064-3406690 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T188215 NZ_CP090364:c3030424-3030131 [Photobacterium sp. TLY01]
ATGGCTGTCGAATTCATAGACACGTGGCTGGAAGCGTTTTACGAGGATGACCAAAGTCACAGGAAGATACCCAGCACCAT
CGAGAGCGCATTGTTCAGGAAGCTGGAAATCTTGGACGCAGCGACCCAGGAGTCAGACTTGCTAATCCCTCCGGGTAACC
GCTTCGAGCACCTTGAAGGAAATATGGCCGGGTGGTGTTCCATCCGGGTAAACAAGCAATACCGGCTGATCTTCAGATGG
ATCGATGGCGTTGCCAAAGATACCTATCTGGACCCTCATGTTTACAGGGGCTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT188215 WP_001295723.1 NZ_CP068812:c3407522-3407154 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT188215 NZ_CP090364:c3030116-3029787 [Photobacterium sp. TLY01]
ATGAGAAACACGAAACGTCGCCCAGTTACGGTCGGTCAGATGCTGGTAACCGAGTTCCTGAAGCCTATGAACATTGAGAT
CAACGAACTGGCTGAGGCTATGGGTGTACACAGAAACACCCTGAGTCGGATCGTTCACGACAAGGGGGCATTGACCGCGC
CCATGGCAATCAAGCTGGCTGCGGCCTTGGGTAATACGCCGGAGTTCTGGCTCAATATTCAGCACTCAGTAGAGTTGTGG
GATGTTCGGCATAGAGCCTTTGAGCAGGAAGCAAAAAATGTTAAGAGGTTAAAGCCGAATCTGGAGCATAGAGAAGTCGC
TTTTCACTAG

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52