Detailed information of TA system TA188212

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2809554..2810386
• Replicon: chromosome
• Accession: NZ_CP068812
• Organism: Escherichia coli strain RIVM_C029020

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: E3PN67
• Locus tag: JNN61_RS13425
• Protein ID: WP_000854775.1
• Coordinates: 2810012..2810386 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A426P7N5
• Locus tag: JNN61_RS13420
• Protein ID: WP_001285645.1
• Coordinates: 2809554..2809922 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN61_RS13385	2805377..2806057	+	681	WP_001282911.1	WYL domain-containing protein	-
JNN61_RS13390	2806208..2806885	+	678	WP_001097567.1	hypothetical protein	-
JNN61_RS13395	2806891..2807124	+	234	WP_001278284.1	DUF905 family protein	-
JNN61_RS13400	2807214..2808032	+	819	WP_001175173.1	DUF945 domain-containing protein	-
JNN61_RS13405	2808124..2808609	+	486	WP_000213717.1	antirestriction protein	-
JNN61_RS13410	2808625..2809101	+	477	WP_001186768.1	RadC family protein	-
JNN61_RS13415	2809170..2809391	+	222	WP_000692303.1	DUF987 domain-containing protein	-
JNN61_RS13420	2809554..2809922	+	369	WP_001285645.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN61_RS13425	2810012..2810386	+	375	WP_000854775.1	TA system toxin CbtA family protein	Toxin
JNN61_RS13430	2810383..2810874	+	492	WP_000976825.1	hypothetical protein	-
JNN61_RS13435	2810886..2811083	+	198	WP_000772035.1	DUF957 domain-containing protein	-
JNN61_RS13440	2811168..2812010	+	843	WP_001274548.1	DUF4942 domain-containing protein	-
JNN61_RS13445	2812194..2812376	+	183	WP_177309578.1	hypothetical protein	-
JNN61_RS13450	2812804..2813988	+	1185	WP_001172876.1	sugar efflux transporter	-
JNN61_RS13455	2814099..2815022	+	924	WP_000535949.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13965.02 Da
• Isoelectric Point: 8.2830

>T188212 WP_000854775.1 NZ_CP068812:2810012-2810386 [Escherichia coli]
MKTLPDTLVREASRCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T188212 NZ_CP090356:c3990725-3990393 [Pantoea ananatis]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTAGATCCGAATGCCGGGCATGAACAAAGCGGAAGGCGTCCAGTACTGAT
TGTTTCTCCGGCGTCCTTTAATCAGTTTACGCGGCTACCTGTGGTTGTTCCCGTGACCAACGGCGGAAACTTTGCCCGCG
CAGCGGGGTTTACTGTTTCACTGGATGGCGCAGGAACGAAAACAACGGGGGTAATTCGTTGTGACCAGCCCAGAACCATT
GATATGGAAGCCCGGAACGGCAAATATCTTGAACGAATACCTGAAGTTATTGTGAATGAAGTGGTGGCCAGACTGGAGGC
CATTCTGAGCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13678.37 Da
• Isoelectric Point: 5.4492

>AT188212 WP_001285645.1 NZ_CP068812:2809554-2809922 [Escherichia coli]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRADIRGRFSDADAYHLDQAFPLLMKQLELMLTNG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT188212 NZ_CP090356:c3990983-3990726 [Pantoea ananatis]
ATGTATACCACTCGTCTGAAAAAGGTTGGCGGATCTGTCATGCTGGCGGTCCCTCCCGCCGTGCTGAAAACGCTGGGGCT
GTCGACGGACAGCGAAGTGGGCATGACTATTGATAGTGGCTGCCTCATAATTGAACCTCAAAAACGTCCCCGCTATTCAC
TCGAAGAACTACTGGCGCAGTGCGATCCGCACGCTGAAATAAGCGAAGAGGGTCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGCTCCTGTAG

Structures

Toxin

• AlphaFold DB: E3PN67

Antitoxin

• AlphaFold DB: A0A426P7N5