Detailed information of TA system TA188005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 941454..942104
• Replicon: chromosome
• Accession: NZ_CP068806
• Organism: Escherichia coli strain RIVM_C018576

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JNN57_RS04400
• Protein ID: WP_000263532.1
• Coordinates: 941454..941795 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U9YXQ7
• Locus tag: JNN57_RS04405
• Protein ID: WP_000212552.1
• Coordinates: 941805..942104 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN57_RS04375	936489..937589	+	1101	WP_001181876.1	flagellar basal body P-ring protein FlgI	-
JNN57_RS04380	937589..937888	+	300	WP_000867281.1	rod-binding protein	-
JNN57_RS04385	938064..939440	+	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
JNN57_RS04390	939455..940384	+	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
JNN57_RS04395	940401..941378	+	978	WP_001195938.1	hypothetical protein	-
JNN57_RS04400	941454..941795	+	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JNN57_RS04405	941805..942104	+	300	WP_000212552.1	helix-turn-helix transcriptional regulator	Antitoxin
JNN57_RS04410	942162..943004	-	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
JNN57_RS04415	943490..944404	+	915	WP_000949083.1	lateral flagellin LafA	-
JNN57_RS04420	944609..945925	+	1317	WP_000609678.1	flagellar filament capping protein FliD	-
JNN57_RS04425	945948..946340	+	393	WP_000285322.1	flagellar export chaperone FliS	-
JNN57_RS04430	946345..946656	+	312	WP_000415004.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T188005 WP_000263532.1 NZ_CP068806:941454-941795 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T188005 NZ_CP090196:2477676-2477963 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11004.86 Da
• Isoelectric Point: 9.7665

>AT188005 WP_000212552.1 NZ_CP068806:941805-942104 [Escherichia coli]
MATLKELMAKQSPESQVRIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT188005 NZ_CP090196:2477437-2477676 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: U9YXQ7