Detailed information of TA system TA187887

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4833264..4834098
• Replicon: chromosome
• Accession: NZ_CP068801
• Organism: Escherichia coli strain RIVM_C018220

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: JNN52_RS23615
• Protein ID: WP_000854820.1
• Coordinates: 4833721..4834098 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: JNN52_RS23610
• Protein ID: WP_001285610.1
• Coordinates: 4833264..4833632 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN52_RS23575	4828985..4829665	+	681	WP_021515201.1	WYL domain-containing protein	-
JNN52_RS23580	4829816..4830493	+	678	WP_060579144.1	hypothetical protein	-
JNN52_RS23585	4830499..4830732	+	234	WP_000883181.1	DUF905 family protein	-
JNN52_RS23590	4830822..4831640	+	819	WP_202847426.1	DUF945 domain-containing protein	-
JNN52_RS23595	4831906..4832385	+	480	WP_000706978.1	antirestriction protein	-
JNN52_RS23600	4832400..4832876	+	477	WP_001186193.1	RadC family protein	-
JNN52_RS23605	4832963..4833184	+	222	WP_000692347.1	DUF987 domain-containing protein	-
JNN52_RS23610	4833264..4833632	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN52_RS23615	4833721..4834098	+	378	WP_000854820.1	type IV toxin-antitoxin system toxin CbtA	Toxin
JNN52_RS23620	4834095..4834292	+	198	Protein_4628	hypothetical protein	-
JNN52_RS23625	4834320..4834517	+	198	WP_085949158.1	DUF957 domain-containing protein	-
JNN52_RS23630	4834602..4835162	+	561	Protein_4630	DUF4942 domain-containing protein	-
JNN52_RS23635	4835997..4837535	+	1539	WP_001187178.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4799974..4845348	45374

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T187887 WP_000854820.1 NZ_CP068801:4833721-4834098 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T187887 NZ_CP090095:c1046458-1046210 [Neorhizobium galegae]
TTGAAGATCATTTGGGACGAGACGAAGAGGATTGTGAATCTCGAGAAGCATGGTCTCGATTTCGCCGATCTTTATTTCGA
ATTCTTCGCAACGGCGGTGACCACACCCGCCAAGAAGGCGAGATCCAAAGCTGTCGGCCGCCTGAAGGATGGAACGATCG
TCGTGATATTCCTGGCGCTCGGCTCGGAAGCGATCTCGGTCATTTCGATGCGCCCTGCCCGAAAAGATGAGAGGAGCATG
ATCGAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT187887 WP_001285610.1 NZ_CP068801:4833264-4833632 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT187887 NZ_CP090095:c1046213-1045899 [Neorhizobium galegae]
ATGAACATCAAGCACGAAAAACAGAAAGAATTTCGCCCTGGTCGCGGATATACCAAAGAGGATTGGGACGCGGTCGACAG
CCCACCACTGACCGCCGAAGAGATGGCGAGCATGCGGCCGTTCCGGGAAGTTTTCCCCGAGATGGCCGCGAAGATGGAAC
AGGCGATCGCCGCCCGTGGCCGGCCGAAACTTGAGGCGCCGAAGGTGGCCGTCACGCTCCGGCTCGATCCGGACGTGCTC
GAAAAGTTCAAGGCGACGGGCAAGGACTGGCGGGCCAAGATGACCGAGGAGCTGCGCAAGGCAGCCGGGCTCTGA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6