Detailed information of TA system TA187866

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2054822..2055653
• Replicon: chromosome
• Accession: NZ_CP068801
• Organism: Escherichia coli strain RIVM_C018220

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: JNN52_RS09930
• Protein ID: WP_000854814.1
• Coordinates: 2054822..2055196 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B3Y195
• Locus tag: JNN52_RS09935
• Protein ID: WP_001285585.1
• Coordinates: 2055285..2055653 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN52_RS09890	2050218..2051384	+	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
JNN52_RS09895	2051503..2051976	+	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
JNN52_RS09900	2052174..2053232	+	1059	WP_001200891.1	FUSC family protein	-
JNN52_RS09905	2053404..2053733	+	330	WP_000450409.1	DUF496 family protein	-
JNN52_RS09910	2053834..2054016	-	183	WP_001016348.1	hypothetical protein	-
JNN52_RS09915	2054088..2054216	+	129	Protein_1942	transposase domain-containing protein	-
JNN52_RS09920	2054505..2054618	-	114	WP_001161660.1	DUF957 domain-containing protein	-
JNN52_RS09925	2054631..2054825	-	195	WP_000988600.1	hypothetical protein	-
JNN52_RS09930	2054822..2055196	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
JNN52_RS09935	2055285..2055653	-	369	WP_001285585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
JNN52_RS09940	2055727..2055948	-	222	WP_000692323.1	DUF987 domain-containing protein	-
JNN52_RS09945	2056011..2056487	-	477	WP_001186726.1	RadC family protein	-
JNN52_RS09950	2056503..2056982	-	480	WP_000860087.1	antirestriction protein	-
JNN52_RS09955	2057064..2057882	-	819	WP_001234629.1	DUF945 domain-containing protein	-
JNN52_RS09960	2057982..2058215	-	234	WP_063610033.1	DUF905 family protein	-
JNN52_RS09965	2058294..2058749	-	456	WP_000581502.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T187866 WP_000854814.1 NZ_CP068801:c2055196-2054822 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T187866 NZ_CP090068:c49264-48971 [Acinetobacter sp. SH20PTE14]
ATGACTTATAAGCTTTTACGTCACAAGGACTTTACAGCAGAATGGGAAAGACTCCCTGTTGCTATACGAGATCAATTTAA
GAAGAAACTAGCCAAAGTCATAGAGCAGCCACATATTCCAAAGAATATGCTTAGAGGCGATCTTGCAGGTTGCTATAAAA
TTAAATTATTAAAAGCTGGTGTCAGACTTGTCTATCAAGTTAAAGATGATCAGGTAGTTATCTTGCTCATCACAGTCGGG
AAACGAGCTGATAGCATAGTTTATGACGAAGCTAAAAAGCGCATTAAAGACTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.48 Da
• Isoelectric Point: 6.3139

>AT187866 WP_001285585.1 NZ_CP068801:c2055653-2055285 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT187866 NZ_CP090068:c49499-49254 [Acinetobacter sp. SH20PTE14]
ATGGCTACTGTTAACTTCAGGGTCGATGAAGCCCTTAAAGAAAAATCCTATTCAATCCTTAAAGAACAAGGTATTGCACC
TACAGATTTCTTTACTAGTATTCTTGAGTATGTTGCTACGACTGGGAAACTACCTGTCAAAAAAGCTTTGCTTTCAGAAG
AAGACGAAGAATTATTAGCTCTTGTCCGTAAGCGCATTAATGACCCTAAAGAGATGTTTGAGGAAGTCACATTAGATGAC
TTATAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A7U9LW60