Detailed information of TA system TA187861

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 869064..869898
• Replicon: chromosome
• Accession: NZ_CP068801
• Organism: Escherichia coli strain RIVM_C018220

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: JNN52_RS04135
• Protein ID: WP_000854690.1
• Coordinates: 869064..869441 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1P7N8
• Locus tag: JNN52_RS04140
• Protein ID: WP_001305076.1
• Coordinates: 869530..869898 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN52_RS04105	865444..865614	-	171	Protein_805	IS110 family transposase	-
JNN52_RS04110	866085..866978	-	894	WP_021528665.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JNN52_RS04115	866971..867366	-	396	WP_000208382.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
JNN52_RS04120	867435..868280	-	846	WP_001529401.1	DUF4942 domain-containing protein	-
JNN52_RS04125	868365..868562	-	198	WP_000839293.1	DUF957 domain-containing protein	-
JNN52_RS04130	868579..869067	-	489	WP_000761699.1	hypothetical protein	-
JNN52_RS04135	869064..869441	-	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
JNN52_RS04140	869530..869898	-	369	WP_001305076.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN52_RS04145	869948..870592	-	645	WP_000094916.1	hypothetical protein	-
JNN52_RS04150	870611..870832	-	222	WP_000692329.1	DUF987 domain-containing protein	-
JNN52_RS04155	870895..871371	-	477	WP_001186726.1	RadC family protein	-
JNN52_RS04160	871387..871872	-	486	WP_000849565.1	antirestriction protein	-
JNN52_RS04165	871927..872745	-	819	WP_001234628.1	DUF945 domain-containing protein	-
JNN52_RS04170	872845..873078	-	234	WP_063610033.1	DUF905 family protein	-
JNN52_RS04175	873157..873612	-	456	WP_000581502.1	hypothetical protein	-
JNN52_RS04180	873688..874815	-	1128	Protein_820	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T187861 WP_000854690.1 NZ_CP068801:c869441-869064 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T187861 NZ_CP090067:463208-463594 [Acinetobacter sp. SH20PTE14]
ATGGATAATCGGCAATTCAAACTGAAACGCAGTTTTTTATCGCATGCGTTTCAGTTTTTTATTTTTGCGGTATTGATGAC
GCTGCTTTATCAGTTACTAACACCCATGCTTTGGGGGATATTTTTCCTCATTGGACTAGTGGTTTATGTACTGTTTTTCC
GTAAGCATTTACACGTCACCAGTCTAGATTATTTGGATGGAACTGATTGGTCTTTGCAACGGCTCAACCGTCCAGTGCAA
CGCGTTCAAATTAGCCATATTTTGGATCATCAAATGTATATCGTGGTGTATTTTATGCATGCGCGTGCAAAACCAGTGAT
TATTTGGCGCGATCAATTGTCATTGAAACAGTGGAAATCACTCAAAGTATTGGCCAAAATGATCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13560.39 Da
• Isoelectric Point: 4.7830

>AT187861 WP_001305076.1 NZ_CP068801:c869898-869530 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT187861 NZ_CP090067:462958-463215 [Acinetobacter sp. SH20PTE14]
ATGACTGATGAAATCAGCTTAGAAGAGCGTAAAGTGATCTACCGCGCACGTCGTGGTTTAAAAGAGATTGATGTTTATTT
TGATCCCTACGTCAAAAATTACTATTTACAAGCCGATGCAGCTGAAAAAGACATGTTTGCAGAACTCGTTGCTGCAGAAG
ATCCAGATTTGTTGGATTGGTTTATGGAAGTCAGTGAACCACCACGTCCTGAATTAAAAACCTTTATTCAAAAATTAAAA
CACTACGTTCATGGATAA

Structures

Toxin

• AlphaFold DB: S1PLF5

Antitoxin

• AlphaFold DB: S1P7N8