Detailed information of TA system TA187265

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 299014..299210
• Replicon: chromosome
• Accession: NZ_CP068553
• Organism: Clostridioides difficile strain 2016

Toxin (Protein)

• Gene name: CD1418.2
• Uniprot ID: D5Q3X5
• Locus tag: JMW46_RS01410
• Protein ID: WP_003424190.1
• Coordinates: 299058..299210 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	CD630_n00500
Locus tag	-
Coordinates	299014..299114 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMW46_RS01395	294281..294538	-	258	WP_003424182.1	hypothetical protein	-
JMW46_RS01400	294525..294758	-	234	WP_003424184.1	hypothetical protein	-
JMW46_RS01405	294946..295134	+	189	WP_003424185.1	helix-turn-helix transcriptional regulator	-
-	299014..299114	+	101	NuclAT_0	-	Antitoxin
JMW46_RS01410	299058..299210	-	153	WP_003424190.1	hypothetical protein	Toxin
JMW46_RS01415	299730..299972	+	243	WP_003424191.1	hypothetical protein	-
JMW46_RS01420	300038..300583	+	546	WP_077699404.1	site-specific integrase	-
JMW46_RS01425	301128..301973	+	846	WP_003424194.1	DUF4878 domain-containing protein	-
JMW46_RS01430	302197..302361	-	165	WP_003424195.1	hypothetical protein	-
JMW46_RS01435	302893..303224	-	332	Protein_286	carboxymuconolactone decarboxylase family protein	-
JMW46_RS01440	303484..304125	-	642	WP_003424198.1	SdpI family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	ant(6)-Ia / tet(40)	-	75904..381030	305126

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5744.78 Da
• Isoelectric Point: 10.6276

>T187265 WP_003424190.1 NZ_CP068553:c299210-299058 [Clostridioides difficile]
MDNFLQGILASLVASLIVYLTSKLFKKVKSHSGRSDFSFELEIKFKKNKH

Nucleotide

• Length: 153 bp

>T187265 NZ_CP089780:c2867065-2866670 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Nucleotide

• Length: 101 bp

>AT187265 NZ_CP068553:299014-299114 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTAGACGTAGAGTGAAGTTCAAATACTAATGTTTATTCTTTTTGAACTTGATTTCTAGTTC
AAAACTAAAGTCACTCCTGCC

Structures

Toxin

• AlphaFold DB: A0A449XCJ1

Antitoxin