Detailed information of TA system TA187212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 660450..660966
• Replicon: chromosome
• Accession: NZ_CP068408
• Organism: Lacticaseibacillus paracasei subsp. tolerans strain S-NA5

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: A0A243Q0H7
• Locus tag: JK381_RS03205
• Protein ID: WP_003573774.1
• Coordinates: 660685..660966 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: JK381_RS03200
• Protein ID: WP_016363783.1
• Coordinates: 660450..660692 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JK381_RS03185	655904..658075	-	2172	WP_003607130.1	class 1b ribonucleoside-diphosphate reductase subunit alpha	-
JK381_RS03190	658247..659236	+	990	WP_003586769.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
JK381_RS03195	659233..659718	+	486	WP_003586772.1	class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI	-
JK381_RS03200	660450..660692	+	243	WP_016363783.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
JK381_RS03205	660685..660966	+	282	WP_003573774.1	type II toxin-antitoxin system YafQ family toxin	Toxin
JK381_RS03210	661166..661564	+	399	WP_003586778.1	DUF1722 domain-containing protein	-
JK381_RS03215	661809..662918	-	1110	WP_003607129.1	hypothetical protein	-
JK381_RS03220	662920..664350	-	1431	WP_003607128.1	hypothetical protein	-
JK381_RS03225	664540..665223	+	684	WP_003586785.1	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	556832..689533	132701
	-	flank	IS/Tn	-	-	654323..655738	1415

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (6-93)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-62)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11371.98 Da
• Isoelectric Point: 9.5081

>T187212 WP_003573774.1 NZ_CP068408:660685-660966 [Lacticaseibacillus paracasei subsp. tolerans]
MNKFVYKPAFERQFKKHYKTILKGGRYKKEDFEAVYHKLLRDEPLDPHYNDHALVNRKPERDLHIKPDWLLIYKYDGEFV
RFIDTGSHSDLFN

Nucleotide

• Length: 282 bp

>T187212 NZ_CP089779:c3552558-3552157 [Mycobacterium tuberculosis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9217.44 Da
• Isoelectric Point: 5.1548

>AT187212 WP_016363783.1 NZ_CP068408:660450-660692 [Lacticaseibacillus paracasei subsp. tolerans]
MTSQVHFRMSEKEKKDFEIVLSRVGLTPGEAFRIFAKKSIEAGGIPFEVSQPNTRLKKAIRSQDYVEFNDPEEGLDWLNE

Nucleotide

• Length: 243 bp

>AT187212 NZ_CP089779:c3552800-3552555 [Mycobacterium tuberculosis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• AlphaFold DB: A0A243Q0H7