Detailed information of TA system TA186784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 2971040..2971733
• Replicon: chromosome
• Accession: NZ_CP068155
• Organism: Escherichia coli strain FDAARGOS_1077

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: I6I34_RS15510
• Protein ID: WP_000415584.1
• Coordinates: 2971040..2971336 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: I6I34_RS15515
• Protein ID: WP_000650107.1
• Coordinates: 2971338..2971733 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I34_RS15475	2966128..2966442	-	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-
I6I34_RS15480	2966473..2967054	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
I6I34_RS15485	2967373..2967705	+	333	WP_077738370.1	DUF2645 family protein	-
I6I34_RS15490	2967751..2969100	-	1350	WP_000673409.1	two-component system sensor histidine kinase QseC	-
I6I34_RS15495	2969097..2969756	-	660	WP_001221493.1	two-component system response regulator QseB	-
I6I34_RS15500	2969908..2970300	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
I6I34_RS15505	2970353..2970835	+	483	WP_000183505.1	transcriptional regulator	-
I6I34_RS15510	2971040..2971336	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
I6I34_RS15515	2971338..2971733	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
I6I34_RS15520	2971866..2973473	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
I6I34_RS15525	2973611..2975869	+	2259	WP_001281866.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T186784 WP_000415584.1 NZ_CP068155:2971040-2971336 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T186784 NZ_CP089772:2202777-2203154 [Mycobacterium tuberculosis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT186784 WP_000650107.1 NZ_CP068155:2971338-2971733 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT186784 NZ_CP089772:2203196-2203645 [Mycobacterium tuberculosis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65