Detailed information of TA system TA186708

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2430148..2430731
• Replicon: chromosome
• Accession: NZ_CP068146
• Organism: Yersinia enterocolitica strain FDAARGOS_1082

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U7FJS5
• Locus tag: I6I39_RS10620
• Protein ID: WP_005162526.1
• Coordinates: 2430453..2430731 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6I39_RS10615
• Protein ID: WP_005162529.1
• Coordinates: 2430148..2430453 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I39_RS10600	2425964..2426704	+	741	Protein_2050	KDGP aldolase family protein	-
I6I39_RS10605	2426834..2428075	+	1242	WP_005162536.1	lactonase family protein	-
I6I39_RS10610	2428148..2430076	+	1929	WP_005162532.1	BglG family transcription antiterminator	-
I6I39_RS10615	2430148..2430453	-	306	WP_005162529.1	HigA family addiction module antidote protein	Antitoxin
I6I39_RS10620	2430453..2430731	-	279	WP_005162526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6I39_RS10625	2430811..2433531	-	2721	WP_005162523.1	magnesium-translocating P-type ATPase	-
I6I39_RS10630	2434303..2435250	+	948	WP_005162520.1	trehalose operon repressor TreR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10694.10 Da
• Isoelectric Point: 7.4011

>T186708 WP_005162526.1 NZ_CP068146:c2430731-2430453 [Yersinia enterocolitica]
MIKSWKHKGLQRFFETGSAAGINPQHVKRIRDRLRFIDLAETIEDVNVAGYKLHPLQGEREGIWSVTVSGNWRITFEFTA
GDAFILNYEDYH

Nucleotide

• Length: 279 bp

>T186708 NZ_CP089762:c4149209-4148925 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10934.77 Da
• Isoelectric Point: 6.7158

>AT186708 WP_005162529.1 NZ_CP068146:c2430453-2430148 [Yersinia enterocolitica]
MKMHNPPHPGEMIEGILEDLGLGIREFARALNVSPSTVQRLVACKAAISPEMAVKLAAVLGSTPAMWLRLQAAYDLGKAE
ENVDVSNLTKLYKPDPISLHS

Nucleotide

• Length: 306 bp

>AT186708 NZ_CP089762:c4149441-4149199 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A7U7FJS5