Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4550857..4551667 Replicon chromosome
Accession NZ_CP068033
Organism Klebsiella pneumoniae strain LYS105A

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag JKA77_RS22145 Protein ID WP_002887280.1
Coordinates 4550857..4551390 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JKA77_RS22150 Protein ID WP_002887278.1
Coordinates 4551401..4551667 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKA77_RS22140 4549688..4550809 + 1122 WP_009309849.1 cupin domain-containing protein -
JKA77_RS22145 4550857..4551390 - 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
JKA77_RS22150 4551401..4551667 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
JKA77_RS22155 4551770..4553203 - 1434 WP_019724905.1 Cu(+)/Ag(+) sensor histidine kinase -
JKA77_RS22160 4553193..4553876 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
JKA77_RS22165 4554048..4555430 + 1383 WP_019724904.1 efflux transporter outer membrane subunit -
JKA77_RS22170 4555448..4555792 + 345 WP_025987660.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T186482 WP_002887280.1 NZ_CP068033:c4551390-4550857 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T186482 NZ_CP089448:2086020-2086123 [Klebsiella michiganensis]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT186482 WP_002887278.1 NZ_CP068033:c4551667-4551401 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT186482 NZ_CP089448:c2086130-2085962 [Klebsiella michiganensis]
AACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTTACCGTGTCAGGTTTTCCAGTCCGCGACAAAAGTG
GACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References