Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 62426..63027 Replicon plasmid pXJW9B263-2
Accession NZ_CP067401
Organism Escherichia coli strain XJW9B263

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag JJF91_RS24400 Protein ID WP_001216045.1
Coordinates 62426..62806 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag JJF91_RS24405 Protein ID WP_001190712.1
Coordinates 62806..63027 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJF91_RS24915 57525..57644 - 120 WP_001376634.1 ash family protein -
JJF91_RS24375 (JJF91_24260) 57866..59350 - 1485 WP_000124150.1 terminase -
JJF91_RS24380 (JJF91_24265) 59350..60543 - 1194 WP_072728159.1 terminase -
JJF91_RS24385 (JJF91_24270) 60630..61082 - 453 WP_001369802.1 hypothetical protein -
JJF91_RS24390 (JJF91_24275) 61171..62214 - 1044 WP_000648825.1 DUF968 domain-containing protein -
JJF91_RS24395 (JJF91_24280) 62242..62421 - 180 WP_000113019.1 hypothetical protein -
JJF91_RS24400 (JJF91_24285) 62426..62806 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
JJF91_RS24405 (JJF91_24290) 62806..63027 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JJF91_RS24410 (JJF91_24295) 63100..63489 - 390 WP_000506726.1 S24 family peptidase -
JJF91_RS24415 (JJF91_24300) 63613..63864 - 252 WP_001283837.1 DNA polymerase III subunit theta -
JJF91_RS24920 63897..64247 + 351 WP_000551789.1 hypothetical protein -
JJF91_RS24425 (JJF91_24310) 64232..64516 - 285 WP_001142394.1 hypothetical protein -
JJF91_RS24430 (JJF91_24315) 64500..65150 - 651 WP_216778061.1 hypothetical protein -
JJF91_RS24435 (JJF91_24320) 65132..65506 - 375 WP_033553822.1 hypothetical protein -
JJF91_RS24440 (JJF91_24325) 65513..65806 - 294 WP_000268000.1 hypothetical protein -
JJF91_RS24445 (JJF91_24330) 65985..66218 - 234 WP_000517421.1 hypothetical protein -
JJF91_RS24925 66301..66489 - 189 WP_032352565.1 DUF551 domain-containing protein -
JJF91_RS24930 66577..66840 - 264 Protein_74 hypothetical protein -
JJF91_RS24935 67057..67191 - 135 Protein_75 hypothetical protein -
JJF91_RS24455 (JJF91_24340) 67188..67520 - 333 WP_157794728.1 hypothetical protein -
JJF91_RS24460 (JJF91_24345) 67525..67703 - 179 Protein_77 hypothetical protein -
JJF91_RS24465 (JJF91_24350) 67705..67893 - 189 WP_000797279.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..96022 96022


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T186292 WP_001216045.1 NZ_CP067401:c62806-62426 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T186292 NZ_CP089313:c4127366-4127082 [Salmonella enterica subsp. enterica serovar Indiana]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT186292 WP_001190712.1 NZ_CP067401:c63027-62806 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT186292 NZ_CP089313:c4127598-4127356 [Salmonella enterica subsp. enterica serovar Indiana]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTACGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References