Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2584032..2584867 Replicon chromosome
Accession NZ_CP067303
Organism Escherichia coli strain ABW_A32

Toxin (Protein)


Gene name yeeV Uniprot ID A0A1X9TM58
Locus tag JJB23_RS12510 Protein ID WP_000854761.1
Coordinates 2584490..2584867 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag JJB23_RS12505 Protein ID WP_001295723.1
Coordinates 2584032..2584400 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB23_RS12475 (2579425) 2579425..2579970 - 546 WP_000973197.1 bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase -
JJB23_RS23470 (2580122) 2580122..2580295 - 174 Protein_2465 cobalamin biosynthesis protein -
JJB23_RS23475 (2580274) 2580274..2580462 - 189 Protein_2466 IS4 family transposase -
JJB23_RS12480 (2580435) 2580435..2580599 - 165 WP_001128468.1 hypothetical protein -
JJB23_RS12485 (2581023) 2581023..2581268 + 246 Protein_2468 transposase -
JJB23_RS12490 (2581436) 2581436..2582005 - 570 WP_000221554.1 inovirus Gp2 family protein -
JJB23_RS23480 (2582204) 2582204..2582404 + 201 WP_001297219.1 hypothetical protein -
JJB23_RS12495 (2582750) 2582750..2582875 - 126 Protein_2471 Hha/YmoA family nucleoid-associated regulatory protein -
JJB23_RS12500 (2583648) 2583648..2583869 + 222 WP_000692345.1 DUF987 domain-containing protein -
JJB23_RS12505 (2584032) 2584032..2584400 + 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
JJB23_RS12510 (2584490) 2584490..2584867 + 378 WP_000854761.1 TA system toxin CbtA family protein Toxin
JJB23_RS12515 (2584864) 2584864..2585012 + 149 Protein_2475 DUF5983 family protein -
JJB23_RS12520 (2585112) 2585112..2585192 + 81 Protein_2476 hypothetical protein -
JJB23_RS12525 (2585623) 2585623..2587162 - 1540 Protein_2477 IS66-like element ISEc22 family transposase -
JJB23_RS12530 (2587211) 2587211..2587558 - 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -
JJB23_RS12535 (2587555) 2587555..2587959 - 405 WP_000839179.1 transposase -
JJB23_RS23485 (2588040) 2588040..2588264 + 225 Protein_2480 transposase -
JJB23_RS12540 (2588471) 2588471..2588842 + 372 WP_001295631.1 IS110 family transposase -
JJB23_RS23490 (2589113) 2589113..2589478 + 366 WP_001282181.1 EutP/PduV family microcompartment system protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 2581023..2587959 6936


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14205.25 Da        Isoelectric Point: 7.3249

>T186048 WP_000854761.1 NZ_CP067303:2584490-2584867 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T186048 NZ_CP089161:c350362-350096 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT186048 WP_001295723.1 NZ_CP067303:2584032-2584400 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT186048 NZ_CP089161:c350613-350362 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1X9TM58

Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References